DNA transcription

the process by which a sequence of DNA nucleotides is used as a template for the synthesis of an RNA molecule

what is transcription?

messenger RNA
transfer RNA
ribosomal RNA
noncoding RNA

what are the major RNA molecules?

to encode the amino sequences of polypeptides

what is the function of mRNA?

read the mRNA and transfer the appropriate amino acid to the polypeptide chain

function of tRNA

forms ribosomes which read mRNA to make proteins

what does rRNA do?

the entire thing

how much of the chromosome is copied?

the sum of all the RNA molecules produced in a cell

what is a transcriptome?

polymerases

RNA is synthesized by ____

DNA template

RNA synthesis requires a _____

5'-3'

is RNA built 3'-5' or 5'-3' ?

3'-5'

the DNA template is copied in which direction? 3'-5' or 5'-3' ?

3'-5'

what type of exonuclease activity does RNA polymerase NOT have (3'-5' or 5'-3')?

1

which, 1 or 2, is the coding strand?

the complementary strand that is identical to the new RNA

what is the coding strand?

2

which is the template strand (1 or 2)?

it serves as the template for RNA synthesis

what is the purpose of the template strand?

RNA polymerase, DNA, and newly forming RNA

what does the transcription bubble contain?

the sequence of DNA needed for the RNA polymerase to recognize the starting point

what is a promoter?

the sequence of the promoter that is common in most DNA and is recognized by the RNA polymerase

what is a consensus sequence?

initiation
elongation
termination

what are the three transcription stages?

eukaryotic

which, prokaryotic or eukaryotic transcription is more complex?

eukaryotic

which, prokaryotic or eukaryotic transcription has several RNA polymerases?

prokaryotes

mRNA maturation does not occur in ... (prokaryotes or eukaryotes?)

5' cap is added
3' poly tail is added
introns are spliced

what occurs during mRNA processing?

eukaryotes

mRNA maturation only occurs in....

protects mRNA from enzymatic destruction

what does the 3' poly tail do?

to protect mRNA from ribonucleases and bind mRNA to ribosome

the function of the 5' cap is....

exon

which, exon or intron, is the coding segment?

both

which, introns or exons, are transcribed by RNA polymerase?

exons

a mature mRNA is only made up of ...

a specific exon may or may not be included into the mature mRNA

what does alternative processing mean?