Studied by 3 people
what does dna sequencing determine?
precise dna nucleotide base sequence in a length of dna
what is dna profiling?
identifying unique areas of a persons dna to create an individual profile to them
what do non coding regions of dna contain?
variable number tandem repeats
what does somatic gene therapy affect, and does it pass onto offspring?
alters body cells and not passed on
what does germ line gene therapy affect, and does it pass onto offspring?
affects sex cells and is passed on
how have sequencing methods changed over time?
become automated, entire genomes can now be read
what benefits are there of genome wide comparison?
can tailor medicines to individuals, can determine evolutionary relationships
what uses are there of dna profiling?
forensics and medical screening
how to amplify dna?
PCR
what is PCR reaction mixture?
dna fragment, primers, free nucleotides, dna polymerase
what is gene therapy?
replacing a faulty allele with a normal allele
is germ line or somatic therapy permanent?
germ line
what is bioinformatics?
use of computer software to analyse biological data
what are the 3 stages of temperature in PCR?
heat to 95 degrees to break H bonds
cool to 55 to 65 degrees to bind primers
heat to 72 degrees so DNA polymerase can create new DNA strands
changes resulting from gene therapy cannot be passed onto offspring, true or false?
false
what is added to 4 separate tubes in dna sequencing?
single stranded dna, dna polymerase, dna primer, free nucleotides, fluorescently labelled nucleotide
what happens to tubes in chain termination sequencing?
undergo pcr, produce many lengths of dna
what is last stage of dna sequencing?
electrophoresis
define dna sequencing.
working out dna base sequence
what could cause errors in PCR?
temperature damage
not enough primer
how would you describe taq polymerase?
thermostable so doesn’t stop pcr cycle to be renewed
what bond is made by a ligase enzyme?
phosphodiester
what bond is broken in the hottest PCR phase?
H
Note 
Flashcard (100)