DNA Extraction and Analysis Techniques

A collection of flashcards covering key vocabulary and definitions related to DNA extraction and analysis techniques for exam preparation.

QIAGEN Protease

Enzyme that breaks down proteins in blood to aid DNA extraction.

Buffer AL

Lysis buffer that breaks open cells and denatures proteins for DNA extraction.

Ethanol

Used in DNA extraction to precipitate DNA, aiding its binding to the spin column.

Buffer AW1

First wash buffer that removes impurities from DNA during purification.

Buffer AE

Releases purified DNA from the spin column for analysis.

Agarose Gel Electrophoresis

Technique used to separate DNA fragments based on size using an electric field.

DNA Ladder

Size reference in gel electrophoresis that estimates the sizes of unknown DNA fragments.

NanoDrop Spectrophotometer

Device used to measure DNA concentration and purity ratios.

ExoProStar

Enzyme mix that purifies PCR products by degrading unused primers and nucleotides.

Ligation

Process of joining two DNA fragments together using ligase enzyme.

Restriction Endonuclease

Enzyme that cuts DNA at specific sequences for analysis or cloning.

Aseptic Techniques

Methods used to prevent contamination when working with microbial cultures.

Competent Cells

Bacterial cells that are treated to allow uptake of plasmid DNA.

Transformation

Process of introducing foreign DNA into a bacterial cell.

TE Solution

Tris-EDTA solution used to rehydrate DNA for storage.

Spectrophotometry

A method to measure the amount of light absorbed by a DNA solution to determine concentration.

PCR (Polymerase Chain Reaction)

Technique used to amplify specific DNA sequences.

Purification

Process of isolating and removing impurities from DNA samples.

S.O.C. Medium

Nutrient medium that helps recover bacteria after DNA transformation.

Blue/White Screening

Method to differentiate between bacterial colonies containing plasmid with or without an insert.

DNA Extraction from Saliva

Process of isolating DNA from saliva samples using specific reagents.