BIOCHEM LABORATORY

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Precipitation and Coagulation

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Precipitation and Coagulation

What are the two tests to isolate proteins from samples?

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Native Confirmation

Proteins assume a specific three-dimensional structure called __

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Globular (spherical)

The native confirmation of Albumin is __

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Fibrous (elongated)

The native confirmation of Gelatin is __

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Denaturation

This is the change in protein native conformation; the disruption and unfolding of protein

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Reversible and Irreversible

What are the two types of denaturation?

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Coagulation

This refers to the clumping of particles together in a solution

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Alcohol and Heat

What are the two causes of coagulation?

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Coagulum

What is the result of coagulation?

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Solids clumping together

What is the appearance/result of coagulation?

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Ethanol and Heating

These materials were used to observe the coagulation of egg albumin

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Precipitation

The refers to the sticking of molecules together to form a solid that will come out from the solution

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Heavy metals, Strong mineral acids, and Alkaloidal reagents

What are the causes of precipitation in egg albumin?

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Coagulation by Heat/Alcohol

This result is due to?

<p>This result is due to?</p>
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Precipitation by Heavy Metal Salts

This is a result due to?

<p>This is a result due to?</p>
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Silver Nitrate (AGNO3)

The reagent used for this test is?

<p>The reagent used for this test is?</p>
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Precipitation by Heavy Metal Salts

This is a result of?

<p>This is a result of?</p>
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Cupric Sulfate (CuSO4)

The reagent used for this test is?

<p>The reagent used for this test is?</p>
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Precipitation by Strong Mineral Acids

This is a result of?

<p>This is a result of?</p>
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Heller’s Test

The test for this reaction is called?

<p>The test for this reaction is called?</p>
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Nitric Acid (HNO3)

What reagent was used for this test?

<p>What reagent was used for this test?</p>
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Precipitation by Strong Mineral Acids

This is a result of?

<p>This is a result of?</p>
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Robert’s Test

The test for this reaction is called?

<p>The test for this reaction is called?</p>
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Conc. Nitric Acid + Sat. Magnesium Sulfate (HNO3 + MgSO4)

What reagent was used for this test?

<p>What reagent was used for this test?</p>
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Precipitation by Alkaloidal Reagents

This is a result of?

<p>This is a result of?</p>
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Picric Acid

The reagent used for this test is?

<p>The reagent used for this test is?</p>
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Precipitation by Alkaloidal Reagents

This is a result of?

<p>This is a result of?</p>
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Tannic Acid

The reagent used for this test is?

<p>The reagent used for this test is?</p>
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Color Reaction

This is a chemical reaction that is used to transform colorless chemical compounds into colored derivatives which can be detected visually.

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Biuret Test

This is a test for peptide bonds.

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Positive

Biuret Test: Is this a positive or negative result?

<p>Biuret Test: Is this a positive or negative result?</p>
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Negative

Biuret Test: Is this a positive or negative result?

<p>Biuret Test: Is this a positive or negative result?</p>
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Ninhydrin Test

This is a test for proteins based on free alpha amino groups.

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Positive

Ninhydrin Test: Is this a positive or negative result?

<p>Ninhydrin Test: Is this a positive or negative result?</p>
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Negative

Ninhydrin Test: Is this a positive or negative result?

<p>Ninhydrin Test: Is this a positive or negative result?</p>
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Xanthroproteic Test

This is a test for phenyl-containing amino acids.

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Phenyl-containing Amino Acids

What does this test indicate?

<p>What does this test indicate?</p>
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Adamkiewicz Test

This is a test for the presence of tryptophan.

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Tryptophan

What does this test indicate?

<p>What does this test indicate?</p>
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Reduced Sulfur Test

This is a test for the presence of cysteine.

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Cysteine

What does this test indicate?

<p>What does this test indicate?</p>
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Molisch Test

This is a test for the presence of carbohydrates in solution.

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Carbohydrates

What does this test indicate?

<p>What does this test indicate?</p>
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Diluted Sodium Hydroxide (NaOH)

Among these reagents, which is yeast RNA fully soluble with?

<p>Among these reagents, which is yeast RNA fully soluble with?</p>
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Hot Water

Among these reagents, which is yeast RNA slightly soluble with?

<p>Among these reagents, which is yeast RNA slightly soluble with?</p>
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Cold Water, 95% Ethanol, Hydrochloric Acid (HCl)

Among these reagents, which is yeast RNA insoluble with?

<p>Among these reagents, which is yeast RNA insoluble with?</p>
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Sulfuric Acid (H2SO4)

What solution is needed for yeast RNA hydrolysis?

<p>What solution is needed for yeast RNA hydrolysis?</p>
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Test for Purine

Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?

<p>Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?</p>
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Ammoniacal Silver Nitrate (Ammoniacal AgNO3) + Concentrated Ammonium Hydroxide (NH4OH)

Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?

<p>Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?</p>
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Test for Pentose

Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?

<p>Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?</p>
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Molisch Reagent + Concentrated Sulfuric Acid (H2SO4)

Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?

<p>Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?</p>
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Test for Phosphate

Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?

<p>Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?</p>
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Ammonium Hydroxide (NH4OH) + Nitric Acid (HNO3) + Ammonium Molybdate ((NH4)2Mo7O24)

Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?

<p>Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?</p>
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