BIOCHEM LABORATORY
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53 Terms
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Precipitation and Coagulation
What are the two tests to isolate proteins from samples?
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Native Confirmation
Proteins assume a specific three-dimensional structure called __
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Globular (spherical)
The native confirmation of Albumin is __
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Fibrous (elongated)
The native confirmation of Gelatin is __
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Denaturation
This is the change in protein native conformation; the disruption and unfolding of protein
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Reversible and Irreversible
What are the two types of denaturation?
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Coagulation
This refers to the clumping of particles together in a solution
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Alcohol and Heat
What are the two causes of coagulation?
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Coagulum
What is the result of coagulation?
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Solids clumping together
What is the appearance/result of coagulation?
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Ethanol and Heating
These materials were used to observe the coagulation of egg albumin
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Precipitation
The refers to the sticking of molecules together to form a solid that
will come out from the solution
will come out from the solution
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Heavy metals, Strong mineral acids, and Alkaloidal reagents
What are the causes of precipitation in egg albumin?
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Coagulation by Heat/Alcohol
This result is due to?
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Precipitation by Heavy Metal Salts
This is a result due to?
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Silver Nitrate (AGNO3)
The reagent used for this test is?
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Precipitation by Heavy Metal Salts
This is a result of?
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Cupric Sulfate (CuSO4)
The reagent used for this test is?
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Precipitation by Strong Mineral Acids
This is a result of?
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Heller’s Test
The test for this reaction is called?
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Nitric Acid (HNO3)
What reagent was used for this test?
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Precipitation by Strong Mineral Acids
This is a result of?
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Robert’s Test
The test for this reaction is called?
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Conc. Nitric Acid + Sat. Magnesium Sulfate (HNO3 + MgSO4)
What reagent was used for this test?
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Precipitation by Alkaloidal Reagents
This is a result of?
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Picric Acid
The reagent used for this test is?
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Precipitation by Alkaloidal Reagents
This is a result of?
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Tannic Acid
The reagent used for this test is?
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Color Reaction
This is a chemical reaction that is used to transform colorless chemical compounds into colored derivatives which can be detected visually.
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Biuret Test
This is a test for peptide bonds.
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Positive
Biuret Test:
Is this a positive or negative result?
Is this a positive or negative result?
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Negative
Biuret Test:
Is this a positive or negative result?
Is this a positive or negative result?
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Ninhydrin Test
This is a test for proteins based on free alpha amino groups.
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Positive
Ninhydrin Test:
Is this a positive or negative result?
Is this a positive or negative result?
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Negative
Ninhydrin Test:
Is this a positive or negative result?
Is this a positive or negative result?
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Xanthroproteic Test
This is a test for phenyl-containing amino acids.
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Phenyl-containing Amino Acids
What does this test indicate?
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Adamkiewicz Test
This is a test for the presence of tryptophan.
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Tryptophan
What does this test indicate?
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Reduced Sulfur Test
This is a test for the presence of cysteine.
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Cysteine
What does this test indicate?
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Molisch Test
This is a test for the presence of carbohydrates in solution.
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Carbohydrates
What does this test indicate?
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Diluted Sodium Hydroxide (NaOH)
Among these reagents, which is yeast RNA fully soluble with?
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Hot Water
Among these reagents, which is yeast RNA slightly soluble with?
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Cold Water, 95% Ethanol, Hydrochloric Acid (HCl)
Among these reagents, which is yeast RNA insoluble with?
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Sulfuric Acid (H2SO4)
What solution is needed for yeast RNA hydrolysis?
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Test for Purine
Tests for the Chemical Component of Nucleic Acids:
Based on the result, which test is this?
Based on the result, which test is this?
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Ammoniacal Silver Nitrate (Ammoniacal AgNO3) + Concentrated Ammonium Hydroxide (NH4OH)
Tests for the Chemical Component of Nucleic Acids:
What reagent(s) was/were needed to fulfill this test?
What reagent(s) was/were needed to fulfill this test?
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Test for Pentose
Tests for the Chemical Component of Nucleic Acids:
Based on the result, which test is this?
Based on the result, which test is this?
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Molisch Reagent + Concentrated Sulfuric Acid (H2SO4)
Tests for the Chemical Component of Nucleic Acids:
What reagent(s) was/were needed to fulfill this test?
What reagent(s) was/were needed to fulfill this test?
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Test for Phosphate
Tests for the Chemical Component of Nucleic Acids:
Based on the result, which test is this?
Based on the result, which test is this?
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Ammonium Hydroxide (NH4OH) + Nitric Acid (HNO3) + Ammonium Molybdate
((NH4)2Mo7O24)
((NH4)2Mo7O24)
Tests for the Chemical Component of Nucleic Acids:
What reagent(s) was/were needed to fulfill this test?
What reagent(s) was/were needed to fulfill this test?
Note 
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