Introduction to Microbiology and Chemistry of Life

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74 Terms

1

Microorganisms

Living entities too small to be seen by the naked eye.

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2

Microbiome

Organisms that stably live on humans.

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3

Pathogenic

Disease causing.

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4

Spontaneous generation

The disproven theory that living organisms can arise from non-living matter.

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5

Louis Pasteur

Disproved spontaneous generation, aseptic technique, pasteurization and fermentation.

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6

Robert Koch

German physician, discovered diseases such as anthrax and cholera.

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7

Edward Jenner

British physician, work with cowpox, term immunity and lead to development of vaccines.

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8

Prokaryotes

No nucleus, DNA in cytoplasm, includes bacteria and archaea.

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9

Eukaryotes

DNA in nucleus, membrane around organelles, includes protists, fungi, plants, animals.

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10

Fungi

Eukaryotes, unicellular or multicellular, includes mold and mushrooms, involved in food, pharmaceuticals, decomposers, and pathogens.

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11

Protozoa

Unicellular, usually motile, can be free entities or hosts.

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12

Algae

Photosynthetic eukaryotes that require light, water, and CO2.

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13

Archaea

Cell wall lacks peptidoglycan, found in extreme environments, includes methanogens, halophiles, and thermophiles.

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14

Bacteria

Single celled, includes bacilli and cocci, has a peptidoglycan layer, can be photosynthetic.

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15

Methanogens

Produce methane as a metabolic byproduct and hate oxygen.

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16

Halophiles

Salt lovers.

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17

Thermophiles

Heat loving.

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18

Viruses

Microscopic, acellular entities with a DNA or RNA core and a protein coat, replicate only in living hosts.

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19

Bacteria (Domain)

Prokaryotic, cell wall made of peptidoglycan, reproduces by binary fission, examples include Escherichia coli and Streptococcus.

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20

Archaea (Domain)

Prokaryotic with unique features, cell wall lacks peptidoglycan, reproduces by binary fission, examples include Halobacterium and Methanogens.

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21

Eukarya (Domain)

Eukaryotic, has a nucleus and membrane-bound organelles, more complex than prokaryotes, genetic material is linear DNA within a nucleus, example: humans.

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22

Matter

Anything that has mass.

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23

Atoms

Most basic unit of matter, consisting of protons, neutrons, and electrons.

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Elements

Pure substances made up of only one type of atom (e.g., hydrogen, carbon).

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Acids

Substances that release hydrogen.

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Bases

Accept H⁺ ions in a solution (pH > 7).

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27

Buffers

Substances that minimize changes in pH by absorbing or releasing H⁺ ions.

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28

Organic Molecule

Molecules containing carbon, typically found in living organisms.

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29

Functional Groups

Specific groups of atoms within molecules that influence chemical properties and reactions (e.g., hydroxyl, carboxyl).

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Macromolecules

Large molecules made up of smaller units, such as proteins, carbohydrates, lipids, and nucleic acids.

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Polymer

A large molecule made up of repeating subunits called monomers.

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Protons

Positively charged particles found in the nucleus.

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Electrons

Negatively charged particles that orbit the nucleus.

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Neutrons

Neutral particles found in the nucleus.

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35

Protons

Positively charged

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Neutrons

Neutral particles

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Atomic Number

Number of protons in an atom, which also equals the number of electrons in a neutral atom.

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Mass Number

Sum of protons and neutrons in an atom's nucleus.

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39

Isotopes

Atoms of the same element with different numbers of neutrons.

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40

First electron shell capacity

First shell has 2 electrons.

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Second and third electron shell capacity

Second and third shells can each hold 8 electrons.

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Ionic Bonds

Transfer of electrons between atoms, forming ions. Strong bond in solid form, weaker in water. (e.g., NaCl)

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Covalent Bonds

Sharing of electrons between atoms. Very strong bonds. (e.g., H₂O, CO₂)

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Nonpolar Covalent Bonds

Equal sharing of electrons.

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Polar Covalent Bonds

Unequal sharing of electrons, creating partial charges.

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Hydrogen Bonds

Weak bonds between polar molecules. Important in DNA and protein structures.

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47

Buffers

Maintain pH stability by absorbing excess H⁺ or OH⁻ ions.

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48

Bicarbonate buffer system

Helps maintain a pH around 7.4 in blood.

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49

Dehydration Synthesis

Bonds monomers together by removing water.

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50

Hydrolysis

Breaks down polymers by adding water.

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Carbohydrates

Organic molecules made of carbon, hydrogen, and oxygen (CH₂O).

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Monosaccharides

Simple sugars (e.g., glucose).

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Disaccharides

Two monosaccharides linked (e.g., sucrose).

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Polysaccharides

Long chains of monosaccharides (e.g., starch, glycogen, cellulose).

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Isomers

Same chemical formula but different structural arrangements.

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Storage Polysaccharides for Plants

Starch (made of glucose monomers).

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Storage Polysaccharides for Animals

Glycogen (made of glucose monomers).

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Structural Polysaccharides for Plants

Cellulose (cannot be digested by humans).

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Structural Polysaccharides for Animals

Chitin (found in insect exoskeletons).

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60

Lipids

Composed of glycerol and fatty acids.

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61

Fatty Acids

Long hydrocarbon chains that can be saturated or unsaturated.

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Triglycerides

Three fatty acids attached to a glycerol molecule.

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Saturated Fat

No double bonds, solid at room temp (e.g., butter).

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Unsaturated Fat

One or more double bonds, liquid at room temp (e.g., olive oil).

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Monounsaturated Fat

One double bond.

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Polyunsaturated Fat

Multiple double bonds.

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Trans-Fats

Artificially hydrogenated fats that increase shelf life (e.g., margarine).

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68

Proteins

Made of chains of amino acids.

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Amino Acid Structure

Central carbon, amino group, carboxyl group, hydrogen atom, and R group (side chain).

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70

Protein Structure Levels

Primary: Sequence of amino acids. Secondary: Alpha-helices and beta-sheets formed by hydrogen bonds. Tertiary: 3D shape formed by interactions of side chains. Quaternary: Multiple polypeptide chains interacting.

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Functions of Proteins

Enzymes (e.g., amylase), Structural components (e.g., collagen), Transport (e.g., hemoglobin), Signaling (e.g., hormones).

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Nucleic Acids

Store and transmit genetic information.

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Components of Nucleic Acids

Made of nucleotides, which consist of a sugar, phosphate group, and nitrogenous base.

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74

Location of Nucleic Acids

Found in all living cells and viruses.

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