Small Nonpolar Molecules
Pass more easily through lipid bilayer
Large polar/charged Molecules
Don’t move easily through lipid bilayer
Protein Channels
Part of membrane that helps large, polar, and ion molecules cross membrane
Passive Transport
Diffusion of substances across a membrane with no energy invested
Diffusion
Tendency for molecules to spread out evenly into the available space (Molecules move from high to low concentration)
Dynamic Equilibrium
As many molecules cross membrane in one direction as the other. Equal movement in both directions (no net movement)
Concentration Gradient
The region along which the density of a chemical substance decreases (diffusion moves down/with gradient)
Osmosis
Diffusion of water (solvent) across a selectively permeable membrane
Goes from high to low solute concentration, ending with near equal solute concentration
Isotonic Solution
Solute concentration is the same as the region it is being compared to
No net water movement across the plasma membrane
Hypertonic Solution
Solute concentration is greater than the region is it being compared to
Cell loses water (water moves out when places in ______), so cell gets shriveled and shrunken
Hypotonic Solution
Solute concentration is less than the region it is being compared to
Cell gains water (water moves into cell when placed in a _______), so cell grows and could even burst
Osmoregulation
Control of solute concentrations and water balance, which is a necessary adaption for life in such environments
Contractile Vacuole
Adaptation Paramecium has where it is hypertonic to its pond water environment and this adaptation pumps water out of the cell
How Animal Cells React to Iso/Hypo/Hyper Solutions
Hypotonic- Animal cell will burst
Isotonic- Ideal environment for Animal cell
Hypertonic- Animal cell becomes shriveled
How Plant Cells react to Iso/Hypo/Hyper Solutions
Hypotonic- Plant cell in happy place (where it wants to be) because cell wall prevents cell from bursting creating turgor pressure which allows plant to stand up nice and tall
Isotonic- Plants will start to wild due to lack of turgor pressure (flaccid)
Hypertonic- Plant cell plasmolyzes (dries up) and the cell wall separates from the cell membrane
Facilitated Diffusion
Passive transport aided by proteins. Channe proteins are hallways and carrier proteins opens up one way and then opens up the other way to release molecules
Active Transport
Uses energy (ATP )to move solutes against their gradients, allowing cells to maintain concentrations
Transport Proteins
Required in active transport and are specific to what molecules they move
Sodium Potassium Pump
Energy used and protein is specifically shaped to fill the sodium and potassium
Proton Pump
Helps store energy that can be used for cellular work by pumping out H+ ions requiring energy and creating voltage across membranes
Cotransport
Coupled transport by a membrane protein (active transport of a solute indirectly drives transport of other solutes)
Ex. Sucrose H+ Cotransport (potential energy from high concentration of protons outside of cell used to bring sucrose into cell)
Bulk Transport
Active transport across plasma membrane by endocytosis and exocytosis
Exocytosis
Transport vesicles migrate to membrane, fuse with it, and release their contents (exo → exit)
Endocytosis
Cell takes in macromolecule to form vesicles from plasma membrane (involves different proteins)
3 types: Phagocytosis, Pinocytosis, and Receptor Mediated Endocytosis
Phagocytosis
Cell engulfs a particle in a vacuole and fuses with a lysosome to digest particle (cellular eating)
Pinocytosis
Molecules are taken up when extracellular fluid is gulped into tiny vesicles (cellular drinking)
Receptor Mediated Endocytosis
Binding of ligands (any molecule that binds specifically to a receptor site of another molecule) to receptors trigger vesicle formation
Cell Membrane
Protects cell from surroundings, maintains an internal environment that is different from the external environment, and determines which particles may enter or exit the cell
Phospholipids
Polar/Hydrophilic head and nonpolar/hydrophobic tail
Saturated Fatty Acid Tail
Packed more tightly, more rigid membrane, molecules pass through more easily (better at hot temperatures where membranes are likely to disintegrate)
Unsaturated Fatty Acid Tail
Packed more loosely, more fluid membrane, molecules pass through more easily (better at colder temperatures where cell membrane are usually to rigid, less likely to solidify)
Amphipathic
Molecules that have both hydrophobic and hydrophilic regions
Cholesterol
Vital component of cell membranes, helping it maintain an appropriate level of fluidity by managing space between phospholipids
Prevents extremes (hot→ disintegrate and cold → solidify) by keeping phospholipids an optimal distance from each other
Transmembrane/Integral Protein
Protein that extends across the entire membrane
Glycoprotein
Protein component embedded in the membrane and a carbohydrate protrusion that dangles out away from it (Cell to cell recognition)
Glycolipid
Carbohydrate extensions protruding from phospholipid (cell to cell recognition)
Peripheral Protein
Studded with these on one side of the protein
Fluid Mosiac Model
Cell membrane fluid due to cholesterol and from top view looks like a mosaic
Water Potential
Predicts the direction of water movement (water always moves from an area of high water potential to an area of low water potential)
Pressure Potential
Either 0 or positive (cannot be negative)
Solute Potential
Either 0 or negative (cannot be positive
Adding solute lowers the water potential
What is the water potential of a pure (distilled) water in an open container?
0 (Pressure = 0 and solute concentration = 0)
What is the water potential at dynamic equilibrium
Water potential will be equal for the environment and the cell
What is the ionization constant for NaCl?
2 (breaks apart in water)
Surface Area
Determines the amount of substances that can enter a cell from the outside environment and the amount of waste product that can exit to the environment
Volume
Determines the amount of metabolic activity a cell carries out per unit of time
What happens as a cell grows larger?
Volume increases more rapidly than surface area, so increased metabolic activity/need for resources/rate of waste production and if surface area isn’t large enough compared to volume the cell will be unable to bring in enough nutrients/expel enough waste
A cell with a bigger surface area/volume ratio
Is more efficient
Cells functioning for more absorption will have adaptations to increase surface area (ex. villi in small intestine and root hairs)
Microscopy
Biologists use microscopes and the tools of biochemistry to study cells
Light Microscope
Visible light passed through specimen and then through a glass lense, lense refract/bend light so that the image is magnified about (can only see the largest organelles- nucleus and mitochondria)
Electron Microscope
Uses electrons, more magnified, used to study subcellular structure
Scanning Electron Microscope
Focus a beam of electrons onto the surface of a specimen, providing images that look 3D
Transmission Electron Microscope
Focus a beam of electrons through a specimen (better to study the internal structure of a cell)
Cell Fractionation
Takes apart cells and separates the major organelles from one another
Centrifuge: Spins mixtures at different speeds (lower speeds → larger components pellet)