Specimen Preparation

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13 Terms

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  • Whole blood

  • Bone marrow aspirate

Added directly to the culture medium

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White Blood Cells

Separated (buffy coat) from other blood elements prior to inoculation to culture medium

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  1. Amniotic Fluid (Amniocytes)

    1. Cell pellet

    2. Supernatant

  1. Centrifuged (800-1000rpm) to retrieve viable cells

    1. Inculcated to culture media

    2. used in biochem test for fetal defects

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Solid Tissue

Must be disintegrated or finely minced

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G banding

  • Treatment: Trypsin

  • Stain: Giemsa

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R banding

  • Heated: Phosphate Buffer

  • Stain: Giemsa

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Q Banding

  • Stain: Quinacrine Dihydrochloride (Fluorochrome)

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C banding

  • Treatment: Barium Hydroxide

  • Stain: Giemsa

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NOR-bonding

Stain: Silver Nitrate

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  1. G-light band

  2. G-Dark band

TYPE OF G-Band:

  1. Biologically more significant because they represent the active regions of the chromosome

  2. Contains relatively fewer active genes

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Q Banding

First banding method developed for human chromosomes

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Distal long arm (q) of the Y chromosome

The most fluorescent site in the human genome

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C banding

Useful for determining the presence of dicentric and pseudodicentric chromosome