OSMIUM - ACETONE - HEAT FIXATIVES

Osmium

Pale yellow powder which dissolves in water (6% at 20°)

Osmium

Ultrathin sections in Electron microscope

Osmium

Fixes and stains conjugated fat and lipids black

Osmium

Expensive, volatile, and inhibits hematoxylin

Osmium

1:5 tissue-to-fixative ratio

Flemmings w/ glacial

Most commonly used osmic acid

Flemmings w/ glacial

Nuclear fixative and permanently fixes fat

Flemmings w/ glacial

Prolonged exposure causes conjunctiva and blindness

Flemming’s w/out glacial

Cytoplasmic fixation

Trichloroacetic

Causes swelling on tissues just like glacial

Trichloroacetic

Weak decalcifying agent

Trichloroacetic

Small tse only due to small penetration

Trichloroacetic

Softening effect on dense fibrous tissue

Acetone

Used in ice-cold temperature (-5 to 4)

Acetone

Water-diffusable enzymes (lipases and phosphatases)

Acetone

Fixes brain tissues for rabies

Acetone

Dissolves fat and evaporates quickly

Heat

Thermal coagulation of tissue proteins

Heat

Microwave technique - accelerates fixation, staining, and decalcification.

Heat

Frozen tissue sections and bacteriologic smears