RFLP Analysis

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7 Terms

1
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What does RFLP stand for

Restriction Fragment Length Polymorphism

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What is the purpose of RFLP in DNA analysis

To analyze DNA by comparing fragment lengths

3
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What are restriction sites?

Short DNA sequences (4-8 bases) recognized and cut by restriction enzymes

<p>Short DNA sequences (4-8 bases) recognized and cut by restriction enzymes </p>
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Where are restriction enzymes naturally found?

In bacteria, like EcoRI in E. coli

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Why might multiple enzymes be needed to differentiate DNA samples?

If one enzyme cuts both DNA samples the same way, another enzyme at a different site may help differentiate them

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What is the advantage of focusing on the intergenic region in circular DNA analysis

It can provide more clear information for DNA analysis

<p>It can provide more clear information for DNA analysis </p>
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Why are humans considered diploid?

They have 2 copies of homologous chromosomes for each gene