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what are the four types of biological molecules?
carbhydrates, lipids, proteins and nucleic acids
what elements are found in carbohydrates?
carbon, hydrogen, and oxygen
what elements are found in lipids?
carbon, hydrogen, and oxygen
what elements are found in proteins?
carbon, hydrogen, oxygen, and nitrogen
what elements are found in nucleic acids?
carbon, hydrogen, oxygen, nitrogen, and phosphorus
what is a monomer?
is a smaller unit that combines to make a polymer
what is a polymer?
is a large molecule made up of many repeating units of monomers joined together by chemical bonds
what is the process by which monomers join to form a polymer?
polymerisation
what type of reaction synthesises most polymers?
by condensation reaction
what type of reaction breaks down most polymers?
by hydrolysis reaction
what is a condensation reaction?
a reaction where monomers join together and release a water molecule
what is a hydrolysis reaction?
a reaction where a water molecule is added to break a chemical bond between 2 molecules
what is the ratio of hydrogen to oxygen atoms in a carbohydrate?
the ratio of hydrogen to oxygen is 2:1
what is the general formula for a carbohydrate?
Cn(H20)n
what is the main role of carbohydrates in living organisms?
provide an energy supply for cells
what is the role of glycoproteins in cellular recognition?
help cells identigy each other and communicate
which carboydrates can be used to make nucleic acids?
deoxyribose and ribose
what are the three types of carbohydrates?
monosaccharides, disaccharides and polysaccharides
what is the difference between a monosaccharide and a disaccharide?
a monosaccharide is made up of one subunit, whereas a disaccharide is made up of 2 subunits
name 3 examples of monosaccharides?
glucose, fructose and galactose
what is the main function of disaccharides?
used for transport
what is the main function of polysaccharides?
used for storage
what are monosaccharides?
the simplest form of carbohydrates, also known as simple sugars
what is the general formula for monosaccharides?
(CH2O)n
what is the difference between pentose and hexose sugars?
pentose have 5 carbon atoms whereas hexose have 6 carbon atoms
what is the formula for glucose?
C6H12O6
what are the names of the two glucose isomers?
alpha-glucose and beta-glucose
what is the difference between alpha-glucose and beta-glucose?
the orientation of the hydroxyl group on carbon 1
what is the primary energy source in animals and plants?
glucose
why is glucose a good energy source?
it is soluble and its bonds store lots of energy
what are disaccharides?
carbohydrates formed when two monosaccharides join together
name three examples of disaccharides
maltose, sucrose and lactose
name the monosaccharides found in maltose, sucrose and lactose
maltose = glucose and glucose
sucrose = glucose and fructose
lactose = glucose and galactose
how are disaccharides formed?
formed via condensation reactions so the hydroxyl group reacts with another this forms a glycosidic bond and releases a water molecule
how are disaccharides broken down?
are broken down via hydrolysis reactions so a water molecule is added to break the glycosidic bond to release the monosaccharides
what are polysaccharides?
complex carbohyrates made up of many monosaccharides joined by glycosidic bonds
name three examples of polysaccharides?
starch, glycogen and cellulose
what is the function of starch in plants?
to store excess glucose and release it to supply energy when needed
what are the characteristics of starch that make it a good energy store?
insoluble, large, many side branches, coiled, hydrolysis releases alpha-glucose monomers
what is the function of glycogen in animals?
to excess glucose and release it to supply energy when needed
what is the structure of glycogen?
glycogen consists of highly branched chains of alpha-glucose monomers joined by glycosidic bonds
what are the characteristics of glycogen that make it good as an energy store?
insoluble, compact, more highly branched than starch, large, hydrolysis releases alpha-glucose monomers
what is cellulose?
a polysaccharide that forms the structural component of plant cell walls
what is the function of cellulose?
to provide strength and rigidity to plant cells
describe the structure of cellulose?
is made of beta glucose joined by 1-4 glycosidic bonds everyother beta-glucose monomer is inverted by 180 degrees
what are the good characteristics of cellulose that make it good at forming new cell walls?
made of long straight and unbranched chains, hydrogen bonds between chains and made of micorfibrils
what are the two categories of sugars that can be tested for?
reducing sugars and non reducing sugars
which sugars are classified as reducing sugars?
monosaccharides and some disaccharides like maltose and lactose
which sugars are classified as non-reducing sugars?
some disaccharides such as sucrose and all polysaccharides
how can you test for reducing sugars?
add bendicts reagent to the sample, heat the mixture in a water bath if reducing sugars are present the mixture will change from a blue solution to a brick red precipitate
what does a blue colour indicate in the reducing sugar test?
no reducing sugar is present
what does an orange colour indicate in the reducing sugar test?
a medium concentration of reducing sugar
what are the quantitative methods to determine reducing sugar concentration?
using a colorimeter or filtering and weighting the precipitate
what is the first step to test for non-reducing sugars?
carry out the test for reducing sugars
how can you test for non reducing sugars?
carry out the test for reducing sugars, add the sample to some dilute hydrochloric acid, heat the mixture in a water bath then neutralise the mixture by adding sodium hydrogencarbonate solution and retest the sample using the test for reducing sugars
how can you test for starch?
add iodine solution to the sample and if starch is present the solution will turn from orange to blue-black
are lipids considered polymers?
no they are not made up of long chains so arent polymers
what are the roles of lipids?
energy supply, structural components, waterproofing, insulation and protection
what are phospholipids used for?
used in cell membranes as structural components
what are most lipids made up of?
made of fatty acids combined with an alcohol, usually glycerol
describe the structure of a fatty acid
a fatty acid consists of a carboxyl group (COOH) attached to a hydrocarbon chain (R group)
what is a saturated fatty acid?
has a hydrocarbon chain saturated with hydrogen with no carbon-carbon double bonds
what is an unsaturated fatty acid?
has a hydrocarbon chain with at least one carbon-carbon double bond, causing the chain to kink
what is the difference between monounsaturated and polyunsaturated fatty acids?
monounsaturated fatty acids have 1 carbon-carbon double bond, while polyunsaturated fatty acids have two or more carbon-carbon double bonds
how can you test for lipids?
place sample in a test tube, add ethanol, shake and add distilled water and a milky white emulsion forms if lipids are present
what is the primary function of triglycerides?
used to store energy in animals, plants and some bacteria
what is the structure of a triglyceride?
consists of a glycerol molecule attached to three fatty acid tails
what characteristics of triglycerides that make them good stores of energy?
long hydrocarbon tails, low mass to energy ratio, insoluble and high ratio of hydrogen to oxygen atoms
how are triglycerides synthesised?
via condensation reactions the OH groups on the glycerol and on 3 fatty acids react to release 3 water molecules and form 3 ester bonds
how are triglycerides broken down?
via hydrolysis reactions the addition of three molecules of water breaks the ester bonds to separate the glycerol and fatty acids
what are the components of a phospholipid?
consist of glycerol, phosphate and 2 fatty acid tails
why are phospholipids described as being polar?
the head is hydrophilic and the fatty acid tails are hydrophobic
how do phospholipids arrange themselves in water?
into a double layer (bilayer) with hydrophillic heads facing out and hydrophobic tails facing in
what are the similarities between triglycerides and phospholipids?
both contain glycerol, fatty acid tails and ester bonds, insoluble in water and contain carbon, hydrogen and oxygen
what are the differences between triglycerides and phospholipids?
phospholipids contain phosphate, phospholipids contain 2 fatty acids but triglycerides contain 3 fatty acids, phospholipids are polar but triglycerides arent, phospholipids form bilayers in water, triglycerides dont
what are proteins made up of?
amino acids
what names are given to the monomers, dimers and polymers of proteins?
monomers = amino acids, dimers = dipeptides, polymers = polypeptides
what are the roles of proteins?
enzymes, antibodies, transport, structural components, hormones, muscle contraction
what is the general structure of an amino acid?
a central carbon atom, an amino group, a carboxyl group, a hydrogen atom and an R group
what determines the properties of an amino acid?
the R group
what type of bond joins amino acids together?
peptide bonds
what is a dipeptide?
two amino acids joined by a peptide bond
how are dipeptides synthesised?
via condensation reactions, the OH in the carboxyl group reacts with the hydrogen in the amino group and releases water and forms a peptide bond
how are dipeptides broken down?
via hydrolysis reactions, a water molecule is added which breaks the peptide bond to release the two amino acids
which test can identify proteins?
the biuret test
what does the biuret test detect?
the presence of peptide bonds
how is the biuret test carried out?
place the food sample in a test tube, add an equal volume of biuret solution, if proteins are present the solution will turn from blue to purple
what colour does a blue colour indicate in the biuret test?
no proteins are present
what are the four main levels of protein structure?
primary, secondary, tertiary and quaternary
what holds the primary structure of a protein together?
peptide bonds
what type of bonds are involved in the secondary structure of a protein?
hydrogen bonds
what are the two possible shapes formed by a protein’s secondary structure?
alpha helix and beta pleated sheet
what hold the tertiary structure of a protein together?
hydrogen bonds, ionic bonds, disulfide bridges, hydrophobic and interactions
what are the weak interactions between polar and non polar R groups called?
hydrophobic interactions
what is the quaternary structure of a protein?
two or more polypeptide chains held together by bonds
what are the bonds involved in the quaternary structure?
hydrogen bonds, ionic bonds, disulfide bridges, hydrophobic and interactions
what are non protein groups added to the quaternary structure called?
prosthetic groups
do all proteins have a quaternary structure?
no only some
what is the primary structure of a protein?
the unique sequence of amino acids in the polypeptide chain
what is the secondary structure of a protein?
is the folding of the polypeptide chain into an alpha helix or beta pleated sheet