Lab #6-10: Fecal Analysis (Cram)

What does EPF mean, how do you determine it?

EPF = Eggs per field, determine by searching slide at 10x

What does EPS mean?

Eggs per slide

What does NOS mean, can you just say negative instead?

No ova seen. Don't say negative because some parasites shead intermittently

What is the simplest method of fecal analysis?

Direct smear

You're making a direct smear, which two mediums can you add to a very small amount of feces on your slide?

1. Saline

2. Lugol's iodine (for colourless organisms)

How do you check that your direct smear is thin enough?

Place some writting under the slide and read through it

What are the four quantitative methods of fecal analysis?

1. Modified Wisconsin

2. McMaster

3. Paracount

4. Squeeze test

When doing the Modified Wisconsin method of quantitative fecal analysis, approximately how much feces do you use?

Size of a loonie, 2–5 grams

When doing the Modified Wisconsin method of quantitative fecal analysis, what solution do you use to mix with the sample?

15 mL of sheather's sugar solution

When doing the Modified Wisconsin method of quantitative fecal analysis, how many beakers will you need, why?

Two. One for mixing the sugar solution and feces, one to have a cheesecloth placed over it and the first beaker's contents poured through

When doing the Modified Wisconsin method of quantitative fecal analysis, how much solution from the second beaker (after straining) should you pour into a test tube?

1–2 cm from the top

Can the Modified Wisconsin method be done without a centrifuge, how long would it take?

Yes, it can be done. The cover slip would have to sit on the test tube for 3 hours

When doing the Modified Wisconsin method of quantitative fecal analysis, how should you program the centrifuge?

1200 –1500 rpm for 5–10 minutes

When doing the Modified Wisconsin method of quantitative fecal analysis, after centrifugation what should be done to the test tube before applying the coverslip?

Form a meniscus with sheather's sugar solution

When doing the Modified Wisconsin method of quantitative fecal analysis, how long should you leave the coverslip on the meniscus?

10 minutes

When doing the Paracount or McMaster method of quantitative fecal analysis, how long should the McMaster counting chamber be left after filling for eggs to rise

5 minutes