Microbiology lab Midterm

Which container would you place your used gloves in?

Biohazardous waste

Which container would you put a used coverslip in?

broken glass container

Which container would you place a razor in?

sharps container

Which piece of glassware is the most precise way to measure large amounts of liquid?

graduated cylinder

Which piece of glassware has a progressively smaller circumference from the bottom to the top?

erlenmeyer flask

Which piece of glassware has an equal circumference until the top where the opening has a much smaller circumference?

media bottle

Which piece of glassware has an equal circumference from the top to the bottom and cannot be used for precise measurements?

beaker

Which machine is used to grow and maintain microorganisms at optimal physical conditions?

incubator

Which piece of glassware is used to move liquid culture around an agar plate evenly?

spreader

Which piece of equipment is used to sterilize tools and glassware on the benchtop?

microincinerator

Which piece of equipment is used for visualizing microbes?

microscope

Which tool is used to move microorganisms between cultures and can be repeatly sterilized?

inoculating loop

Which machine rotates a mixture to separate out the different parts?

centrifuge

Which machine is used to heat a mixture and stir a mixture using electromagnetic forces?

hot plate / magnetic stirrer

Which machine agitates a mixture in a test tube to make a homogenized mixture?

vortex mixer

Which machine is used to measure masses?

analytical balance

Which piece of equipment is used to avoid contamenation when working with microbes?

laminar flow hood

Which piece of equipment is used to sterilize equipment and media?

autoclave

Which piece of equipment is measure the amount of material based on absorbance?

spectrophotometer

Which machine is used to perform polymerase chain reactions?

thermocycler

Which machine is used to keep liquids at a constant temperature?

waterbath

Which piece of glassware/plastic is to hold small amounts of liquid in molecular biology?

microcentrifuge tube

Which piece of glassware is used to grow microbes in liquid culture?

test tube

Which piece of glassware is used to grow microbes in solid or semi-solid agar?

petri dish

Which pipet moves a fixed volume?

pasteur pipette

Which pipet is used to transfer the largest amounts of liquid?

serological pipet

Which pipet is used to transfer the smallest amounts of liquid?

micropipette

Those organisms that pose very little risk of disease for healthy students, and they can still capable of causing infection under certain circumstances are rated

BSL-1

Those organisms that pose a moderate risk of infection, but the diseases caused by these organisms are treatable and usually not serious are rated

BSL-2

Though we will not use these organisms during lab, organisms that can cause disease in healthy adults and can spread to the community but have an effective treatment are rated as

BSL-3

Though we will not use these organisms during lab, organisms that can cause disease in healthy adults, poses a lethal risk and does not respond to vaccines or antimicrobial therapies are rated as

BSL-4

Growing microbes in culture is important to increase visibility and manage microbes in an artificial environment is which of the following:

Inoculation

The method uses media to separate out individual microbes to make pure culture is known as:

Isolation

This process that uses media and cultured microbes occurs when the microbes are placed under optimal growth condition to promote growth and reproduction of the microbes is known as:

Incubation

This is the analysis of collected data to help determine the type of microbe that is present and is known as:

Identification

This type of microscope cause the light source to hit the specimen at a severely oblique angle.

darkfield

This is the most common type of microscope used.

brightfield

A benefit of this microscope is the ability to clearly view live organisms without stains.

phase contrast

When using the scanning objective, the objective magnification would be

4x

When using the low-power objective, the objective magnification would be

10x

When using the high-dry objective, the objective magnification would be

40x

When using the oil immersion objective, the objective magnification would be

100x

Using the provided values for multiple microscopes, what is the mean field of view in micrometers for the 4x objective (to three decimal places)?

4,216.667

Using the provided values for multiple microscopes, what is the mean field of view in micrometers for the 10x objective (to three decimal places)?

1,760.417

Using the provided values for multiple microscopes, what is the mean field of view in

micrometers for the 40x objective (to three decimal places)

421.667

Using the provided values for multiple microscopes, what is the mean field of view in micrometers for the 100x objective (to three decimal places)?

176.042

Using the scale bar, what is the size of a single Escherichia coli cell in micrometers using the given picture?

2.5

Using the scale bar, what is the size of a Pseudomonas aeruginosa cell in micrometers using the given image?

3.5

Using the scale bar, what is the size of Staphylococcus epidermidis in micrometers using the provided image?

1

Using the scale bar, what is the size of Bacillus subtilis in micrometers using the provided image?

3

Using the scale bar, what is the size of a single fungal cell in micrometers?

4

Which type of cell wall (Gram negative or Gram positive) was Escherichia coli?

 Gram negative

Which type of cell wall (Gram negative or Gram positive) was Pseudomonas aeruginosa?

 Gram Negative

Which type of cell wall (Gram negative or Gram positive) was Bacillus subtilis?

Gram Positive

Which type of cell wall (Gram negative or Gram positive) was Stahylococcus epidermidis?

1. a)  Gram negative

2. b)  Gram negative

Question 21. Which supergroup do Kinetoplastida, Diplomonads, Parabasilids, Euglenazoans and Euglenids belong to?

a) Alveolata b) Animals c) Excavata d) Rhizaria

Question 22. Which supergroup do Cercozoa, Radiolarians and Foraminiferans belong to?

a) Alveolata b) Animals c) Excavata d) Rhizaria

Question 23. Which supergroup do Apicoplexans, Ciliates and Dinoflagellata belong to?

a) Alveolata b) Animals c) Excavata d) Rhizaria

Question 24. The phylum Nematoda belongs to which kingdom?

a) Alveolata b) Animals c) Excavata d) Rhizaria

Question 25. The phylum Platyhelminthes belongs to which kingdom?

a) Alveolata b) Animals c) Excavata d) Rhizaria