Phlebotomy Tubes

Blood Culture; Additive?, # of Inversions?, Departments?

Additive: Nutrient Broth, Inversions: 3-4, Department: Microbiology

What common tests are blood cultures used for?

culture and sensitivity (C&S)

How many mL of blood do you draw for a blood culture? How much for pediatric cultures?

8-10mL; 1-3mL

Light Blue Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Sodium Citrate, Yield: Plasma, Inversions: 3-4, Department: Chemistry

When using a winged infusion set to collect a blue tube what should you do before inserting tube?

Insert as red discard tube to get rid of air in the butterfly tube.

What are common tests that the light blue tube is used for?

PT, INR, PTT, aPTT, D-Dimer

Red Plastic Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Clot activator, Yield: Serum, Inversions: 5-10, Department: Chemistry

Red Glass Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: None, Yield: Serum, Inversions: 0, Department: Chemistry or Blood Bank

Clear or White Red Stripped; Additive?, Yield?, # of Inversions?, Departments?

Additive: None, Yield: N/A, Inversions: 0, Department: Discard

Gold Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Clot activator and gel separator, Yield: Serum, Inversions: 5-10, Department: Chemistry

Black and Red Stripped Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Clot activator and gel separator, Yield: Serum, Inversions: 5-10, Department: Chemistry

Because of the gold tube and black and red stripped tube additive what should you do with the tube after collecting it?

Let sit for 30-45 minutes in an upright position then centrifuge to separate components

What are some common tests that the gold tube and black and red stripped tube is used for?

CMP, BMP, Lipid Panel, Hepatic Function Panel, Renal Function Panel, Chemistry Serum Determinations

Orange Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Thrombin-based clot activator, Yield: Serum, Inversions: 5-6, Department: Chemistry

Light Green Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Lithium heparin and gel separator, Yield: Plasma, Inversions: 8-10, Department: Chemistry

What are common tests that are used in the light green tube?

STAT chemistry tests, hCG, electrolytes

White and Green Stripped Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Lithium Heparin and gel separator, Yield: Plasma, Inversions: 8-10, Department: Chemistry

Dark Green Tube (LH); Additive?, Yield?, # of Inversions?, Departments?

Additive: Lithium heparin and gel separator, Yield: Plasma or whole blood, Inversions: 8-10, Department: Chemistry

What is the most common test that the dark green sodium heparin tube tests for?

Ammonia

What should you do after collecting the dark green sodium heparin tube?

Place in ice or cold

Dark Green Tube (SH); Additive?, Yield?, # of Inversions?, Departments?

Additive: Sodium heparin, Yield: Plasma, Inversions: 8-10, Department: Chemistry

Lavendar Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: K2 EDTA, Yield: Plasma or whole blood, Inversions: 8-10, Department: Hematology

What are the most common tests a lavender tube test for?

CBC, ESR, H&H, hemoglobin A1c, Ammonia (depends of lab)

What is Hemoglobin?

red-colored, iron rich protein in red blood cells that transports oxygen and carbon dioxide throughout body

What is Hematocrit?

the percentage of red blood cells in a blood sample

Pink Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: K2 EDTA, Yield: Whole Blood, Inversions: 8-10, Department: Blood Bank

What are the most common tests that the pink tube is used for?

Blood typing, crossmatching

White Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: K2 EDTA and gel separator, Yield: Plasma, Inversions: 8, Department: Molecular Diagnostics

Tan Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: K2 EDTA, Yield: Whole Blood or Plasma, Inversions: 8, Department: Chemistry

Gray Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Sodium Fluoride and Potassium Oxalate, Yield: Whole Blood or Plasma, Inversions: 8-10, Department: Chemistry

When drawing a gray tube what should you not use while prepping the area? What should you use instead?

an alcohol cleaner. chlorhexidine or other non-alcohol cleaner

How should you draw a lactic acid test?

without stasis

What are common tests the gray tube is used for?

Glucose, lactic acid, blood alcohol

Royal Blue with Red Strip Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: None, Yield: Serum, Inversions: 0, Department: Chemistry

What are common tests that Royal Blue with Red Strip Tube tests for?

Selenium, Copper

Royal Blue with Lavendar Strip Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: K2 EDTA, Yield: Whole Blood or Plasma, Inversions: 8, Department: Chemistry

What are common tests that Royal Blue with Lavendar Strip Tube tests for?

Zinc, Arsenic, Lead

Yellow SPS Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Sodium polyanethol sulfonate, Yield: Whole Blood, Inversions: 8, Department: Microbiology

Yellow ACD Tube; Additive?, Yield?, # of Inversions?, Departments?

Additive: Acid Citrate Dextrose, Yield: Whole Blood or Plasma, Inversions: 8, Department: Blood Bank

What color tubes are SST, serum separator tubes?

Red, Gold, Tiger, marble

List the order of tube draws.

Blood Culture, Light Blue, Red, Orange, light green, dark green, lavender, pink, white, tan, gray, royal blue, yellow