CHE 445 Plots and Inhibition

Noncompetitive Inhibition (Graph)

Uncompetitive Inhibition (Graph)

Competitive Inhibition

Vm = no change, Km increases

Noncompetitive Inhibition

Km = no change, Vm = decreases

Uncompetitive Inhibition

Decreases Km and Vm

Binding Site - Competitive

only free enzyme, binding site

Binding site - noncompetitive

binds to differnt part of enzyme, not binding site, binds to free enzyme and enzyme substrate complex

binding site - uncompetitive inhibitor

only binds to enzyme substrate complex

Increasing factor

Km’ = Km (1+[I]/Ki) **Only for competitive

Decreasing factor

Vm’ = Vm/(1+[I]/Ki) **both non and un

Km’ = Km/(1+[I]/Ki) **ONLY Un

units for vm

conc/t

units for km

conc.

high substrate concentration

0th order rxn

low substrate concentration

1st order rxn

competitive inhibition graph