Immobilized Enzymes

Enzymes in Bioltechnology

Before scientists were able to just use the enzyme in biotechnology what did they used to use?

Whole Microrganism

How do scientists broadly immobilize the enzyme?

Attatch to insoluble material

What are 4 methods of immobilisaiton?

• Adsorption

• Covalent Bonding

• Entrapment in Matrix

• Membrane Separation

What is adsorption?

Bonding the enzyme to a surface such as clay with ionic bonds and hydrophobic interactions

What is covalent bonding immobilisation?

Bonding the enzyme to a surface such as clay with covalent bonds

What is entrapment in matrix?

Matrix is a material such as collagen preventing the enzyme moving

What is Membrane Separation?

Separate the enzyme from reaction mixture with partially permeble mebrane

Products diffuse out

Substrate diffuse in to reach enzyme

What are advanatges of using immobilised enzymes?

• Reduces downstream processing

• Allows enzyme to be recovered reducing cost

• High Temperature tolerance which increases yield

What are disadvanatges of using immobilised enzymes?

• Its expensive to immobilise

• Enzymes may detach and leak into mixture

• Method may change shape of active site reducing the rate of reaction