(d) the effect of enzyme concentration on the rate of a reactin

What is the method

Make 2% 1% 0.5 % 0.25% 0.13% trypsin by serial dilution

Label 5test tubes with trypsin concentrations

Hold one up toobservation sheet and check you cant see through it

Add cm3 of 2% trypsin to boiling tube and start clock

Hold against o.shet and stop timer when visible

Recor results

Repeat with other concentrations

Plot graph

Why is trypsin used

Protease that catalyses the breakdown of proteins

What are the risks

Milk - possible allergen

2% trypsin - irritant of eye and skin

What are the control variables

Volume of milk powder solution and trypsin concentrations

Person determining end point

Always done under same conditions

How woul you improve the validity of the results

Check PH to check same

Repeat3 times and take mean

What is the conclusion

Oncentration of trypsin decreases as the rate of reaction decreases

Less enzymes to break down the milk and less active sites to bind to the substrate and form enzyme substrate complexes - less substrate broken down at once.