Chapter 2: Blood banking Reagents Overview and Application

A

1. What is the purpose of including a reagent control when interpreting group AB, D-positive red cells after testing with a low-protein anti-D reagent?

a. to detect false-positive agglutination reactions

b. to detect false-negative agglutination reactions

c. to identify a mix up with patient’s sample

d. to confirm ABO typing results

B

2. Monospecific AHG reagents:

a. increase the dielectric constant in-vitro

b. contain either anti-IgG or anti-C3d antibody specificities

c. are not useful in identifying the molecule causing a positive DAT

d. contain human IgG or complement molecules

A

3. You have added IgG-sensitized red cells to a negative indirect antiglobulin test.

You observe agglutination in the tube. What situation was not controlled for in testing by adding these control cells?

a. the addition of patient serum

b. the addition of AHG reagent

c. adequate washing of cell suspension

d. adequate potency of AHG reagent

C

4. Part of the daily quality control in the blood bank laboratory is the testing of reagent antisera with corresponding antigen-positive and antigen-negative red cells.

What does this procedure ensure?

a. antibody class

b. antibody titer

c. antibody specificity

d. antibody sensitivity

D

5. Group O red cells are used as a source for commercial screening cells because:

a. anti-A is detected using group O cells

b. anti-D reacts with most group O cells

c. weak subgroups of A react with group O cells

d. ABO antibodies do not react with group O cells

C

6. Information regarding reagent limitations is located in the:

a. SOPs

b. blood bank computer system

c. product inserts

d. product catalogs

B

7. What regulatory agency provides licensure for blood banking reagents?

a. AABB

b. FDA

c. American Red Cross

d. College of American Pathologists

C

8. What antibodies are present in polyspecific AHG reagent?

a. anti-IgG

b. anti-IgM and anti-IgG

c. anti-IgG and anti-C3d

d. anti-C3d

A

9. In which source are the regulations regarding the manufacturing of blood banking reagents published?

a. Code of Federal Regulations

b. AABB Standards for Blood Banks and Transfusion Services

c. AABB Technical Manual

d. AABB Accreditation Requirements Manual

B

10. After the addition of anti-D reagent to a patient’s red cell suspension, agglutination was observed. The result with anti-A reagent was negative. What is the interpretation of this patient’s D typing?

a. patient is D-negative

b. patient is D-positive

c. cannot interpret the test

d. invalid result

D

11. What reagent would be selected to detect the presence of unexpected red cell antibodies in a patient’s serum sample? a. A1 and B cells b. panel cells

c. IgG-sensitized cells d. screening cells

A

12. Select the method that uses the principle of sieving to separate larger agglutinates from smaller agglutinates in Ag-Ab reactions.

a. gel technology

b. solid-phase adherence

c. microplate

d. none of the above

B

13. To determine the specificity of a red cell antigen in a patient sample, what source of antibody is selected?

a. commercial reagent red cells

b. commercial antisera

c. patient serum

d. patient plasma

A

14. To determine the presence of a red cell antibody in a patient sample, what source of antigen is selected?

a. commercial reagent red cells

b. commercial antisera

c. patient serum

d. patient’s red cells

C

15. What reagents are derived from plant extracts?

a. panel cells

b. commercial anti-B

c. lectins

d. antiglobulin reagents