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why is the eye so important
larry hester lost his vision from a rare genetic disorder, lead to poor night vision, loss of peripheral vision, and loss of central vision (completely blind). his experience shows the importance of the eye and the retina
what is light
spectrum of electromagnetic energy that ranges from short wavelength gamma rays to long wavelength radiowaves
what is a wavelength
the distance between peaks in the electromagnetic wave

what is the most perceived light
reflected light
what is the visible spectrum for humans
400-700 nanometers
what are the basic components of the eye
pupil, cornea, lens, retina, photoreceptors
what is the pupil
the hole in the center of the eye, an opening where light reflected from objects enters the eye
what is the cornea
transparent focusing “element”, the first structure that light passes through as it enters the eye
what is the lens
transparent focusing “element” where light enters AFTER passing through the cornea
what is the retina
network of cells that covers the back of the eye
what are photoreceptors, and what are the types
receptors of vision, rods and cones
can the lens change shape, and why
yes, it changes shape to focus (accomodation)
what are rods
cylinder shaped photoreceptors that are responsible for dim light (ex: night vision)
what are cones
cone shaped photoreceptors responsible for high illumination vision (daytime), colors, and detailed vision
what is in the inner segment of rods and cones
cell body/soma
what is in the outer segment of rods and cones
contain visual pigments, which are light sensitive chemicals that react to light and trigger electric signals
what is the circuitry of the retina
rods and cones, bipolar cells, and retinal ganglion cells
what are bipolar cells
connections between photoreceptors and retinal ganglion cells
what are retinal ganglion cells
they gather info from multiple bipolar cells and output them to the brain via the optic nerve (optic nerve is RGC axons)
what is the fovea and foveal vision
fovea is a small area of the eye with only cones, foveal vision is for very detailed and colored vision
what is the peripheral retina/vision
peripheral retina is the area outside the fovea and contains both rods and cones. there are more rods than cones, so peripheral vision is for less detailed vision that lacks color
what is the basic function of vision
acuity, the smallest amount of detail you’re able to perceive and tell apart
what happens if the fovea and surrounding areas degenerate
macular degeneration, most common blindness in old people, creates blind region in central vision
what happens if the peripheral retina degenerates
retinitis pigmentosa, degeneration of rod receptors in periphery, cause poor peripheral vision. foveal cones are attacked too, which can result in complete blindness
what is the one area in the retina with no photoreceptors
the optic nerve (RGC axons), this results in a blind spot
how is light focused onto the retina
a two element system, the cornea and the lens
how much does the cornea impact the eye focusing
accounts for 80% of the eye’s focusing power
how much does the lens account for the eyes focusing power
20%, changes shape depending on distance of object using ciliary muscles
what is accomodation
changing of the lens shape to bring objects at different distances into focus
what are refractive errors
errors that happen from the inability of the cornea or lens to properly focus
what is presbyopia
“old eye” age related changes, can’t accommodate for near objects, from lens hardening (weakening of ciliary muscles)
what is myopia
nearsightedness, can’t see distance objects. could be from refractive myopia (cornea/lens bends light too much) or axial myopia (eyeballs too long)
what is hyperopia
farsightedness, can’t see close objects, usually due to short eyeballs
what are the two parts that the light sensitive visual pigments in rods and cones contain
opsin: a long protein, retinal: light sensitive molecule
what happens when visual pigment asborbs light
it isomerizes/changes shape
what happens when visual pigment isomerizes
it creates a chain reaction within the photoreceptor, causing it to go from depolarized (resting state in the dark) to hyperpolarized (from closure of ion channels) called phototransduction
what is the basic function of a sensory system
adaptation, to accommodate for changes luminance levels
what is sensory adaptation
resetting of sensitivity according to the surrounding conditions, this is a dual system of rods and cones that adjusts to RGC outputs depending on levels of surrounding luminance (ex: pirate eyepatches)
what is scotopic vision
vision under dark (night) conditions, not very good color vision, poor acuity, rods are active but cones are not
what is mesopic vision
vision under low light conditions (dawn/dusk), both rods and cones are active
what is photopic vision
vision under high light conditions (daytime) ,good color and acuity, cones are active, rods are not
what does the dark adaptation curve show
when a participant becomes more sensitive to light
what is the difference between light and dark adapted sensitivity
light adapted is your eye adapting to light so it doesn’t blind you while dark adapted is your eye becoming more sensitive to light after spending time in a dim room
how long does it take the rods and cones to reach maximum light sensitivity
cones take 4-6 minutes while rods take 20-30 minutes
how do we measure rod adaptation without cones
using rod monochromats, people who were born with no cones at all, and using rod cone break: the place where rods begin to determine the dark adaptation curve
what are the two parts of visual pigments
opsin, a long protein; and retinal; a chemical that reacts to light to start transduction
what happens when light hits the photoreceptors
visual pigments absorb the light. this causes retinal to straighten out (isomerization), and isomerization creates a chain reaction int he photoreceptor
why does visual pigment regeneration happen
because bleached pigments cannot respond to light
how to visual pigments become bleached
eventually, retinal detaches from opsin, causing the opsin to become lighter (bleaching)
how does visual pigment regeneration work
retinal needs to return to opsin to become functional again
what is the difference in bleached and intact pigments in light and dark
in light, eye has some bleached pigments and some intact pigments, but in the dark, bleached pigments regenerate but don’t isomerize so only intact pigments are present
how long does it take cones and rods pigments to regenerate
cone pigments take 6 minutes while rod pigments take 30 minutes
what is spectral sensitivity
how sensitive our visual receptors are to different parts of the visible spectrum
how is spectral sensitivity measured
by determining the spectral sensitivity curve
how do you measure spectral sensitivity
present light at a single wavelength (monochromatic light) and measure a person’s threshold for viewing different monochromatic light
what is threshold curve for spectral sensitivity
how much light is needed to see the wavelength
what do lower thresholds mean
lower threshold means higher sensitivity
what is the cone spectral sensitivity curve
cones are more sensitive to medium and long wavelength light (most sensitive at 560nm), typically done by presenting light to the fovea
what is the rod spectral sensitivity curve
rods are more sensitive to short-wavelength light (most sensitive at 500nm), typically done in the dark with light presented in the periphery
what is the purkinje shift
The way our eyes switch from seeing bright colors to seeing better in the dark by focusing on more blue-green shades
what is an absorption spectra
a chart that shows which colors of light the rods and cones can absorb the best
why are rods and cones sensitive to different colors
because their pigments absorb different wavelengths of light, this is how we see colors and details in various conditions
what do we notice about the absorption spectra and spectral sensitivity curve when it comes to the rods
rod pigment absorption spectra matches the rod spectral sensitivity curve
what do we notice about the absorption spectra and spectral sensitivity curve when it comes to the rods
the average three cone pigment absorption spectra almost matches the cone spectral sensitivity curve, more M and L cones than S cones
what are horizontal cells
transmit info laterally across the retina, synapse with receptors and bipolar cells
what are amacrine cels
transmit information laterally in the retina, synapse with bipolar cells and RGC
what happens when RGCs recieve info from the different kinds of cells
neural convergence, when many neurons/cells synapse into a single cell
why do rods have stronger neural convergence than cones
rods need less light to generate a response compared to cones, so you don’t need as strong stimulation to activate the RGCs
why is there such high acuity in cones in the context of convergence
there is a lack of neural convergence with cones. rods always stimulate the same RGC no matter if lights hit different nearby rods or spaces between them, cones always stimulate a unique RGC, so since cones don’t mix their signals, they help you see things more clearly
what did H. Keffer Hartline discover and how
RGB receptive fields, he isolated a single RGB axon in the opened eyecup of a frog and found that the axon fired if only a small area of the retina was illuminated
what is the receptive field
the area on the retina that influences the firing rate of the neuron
what did stephen kuffler discover
RGC’s have center-surround receptive fields
how does the RGC receptive field work
stimulation in center is either excitatory or inhibitory, stimulation on the edges causes the cell to react in the opposite way, this happens because of lateral inhibition and it helps the cell to focus better and see details more clearly
what is lateral inhibition
when amacrine and horizontal cells in the retina send signals to reduce the activity of nearby nerve cells
how do center-surround receptive fields help us identify certain details
they make it easier for us to see edges
what is the chevreul illusion
helps edges appear sharper
how is visual acuity at birth
poor, acuity develops fast over the first 6 months, and then slowly until year 1
WHY do infants have such poor acuity at birth
widely spaced and poorly developed cones, poorly developed visual cortex