Microbial Genomics Final Exam second half

What is the a transformasome?

a specialized membranous structure that functions in DNA uptake

Electroporation functions by

making holes in the cell membrane that allow DNA to enter

Where did the name phage come from?

from the Greek word to eat

What nucleic acid material can be in phage?

dsDNA, ssDNA, dsRNA, ssRNA

In the head and tail phages what limits the genome size?

the size of the head

What is lysogeny?

the phase when a phage insert itself into the genome

What is prophage?

a phage inserted into the genome of the cell

What is a lysogen?

a bacterium a phage in its genome

Phage T4 goes through four phases during the infection: immediate early, delayed early, middle, and late. Which is the correct order for the RNA polymerase used by phage T4 for each phase?

E. coli sigma 70 RNA polymerase > antitermination factors > MotA > gp proteins

Phage T4 uses an anti-sigma factor, AsiA, to stop the normal E. coli RNA polymerase from transcribing sigma 70 genes. What is true of how this protein acts?

AsiA binds to sigma 70 on RNA polymerase and prevents from binding to sigma 70 promoters

What does the phage T4 MotA protein do?

Insert itself in RNA polymerase next to sigma 70