phisio 8 pt 2- lab assignment

Which of the following is an end product of starch digestion?

amylase maltose

glucose

amylase and maltose

maltose and glucose

maltose and glucose

The substrate for amylase is

maltose.

starch. carbohydrate.

maltose and starch.

starch and carbohydrate.

starch and carbohydrate

Which of the following is true of enzymes?

• They become part of the product.

• They are used up in the reaction.

• Their activity can be affected by temperature and pH.

• They slow down the rate of the reaction.

their activity can be affected by temp and pH

the reagent IKI taste for the presence

strach

Which of the following is not true of controls?

With a negative control, a negative result is expected.

With a positive control, a positive result is expected.

A positive result with a negative control could mean that a contamination substance is present.

A negative result with a positive control is required to validate the

D

5 Which of the following is an end product of starch digestion?

amylase maltose

glucose

amylase and maltose maltose and glucose

maltose and glucose

Hydrolases are enzymes that break down large molecules into smaller subunits through the addition of

tra

acid

water

salt

base

WATER

tube 2 (amylase, starch, pH 7.0 buffer) appears to have the same amount of starch digested as tube 3 (amylase, starch, pH 7.0 buffer) because

freezing had no effect on the enzyme

Which of the following describes the effect of boiling and

freezing?

Freezing denatured the enzyme, but boiling had no effect.

Boiling and freezing both denatured amylase.

Boiling denatured the enzyme, but freezing had no effect.

Boiling and freezing had no effect on amylase.

boiling denatured the enzyme but freezing had no effct

What does a strong IKI result

indicate?

Substrate is present.

Amylase activity is optimal.

Product of the reaction is present.

Amylase is working.

subtrance is preset

The presence of reducing sugars

_______.

is detected using IKI

means that amylase has been denatured

is detected using Benedict's reagent

means that the substrate is present

is dented using benedicts reagent

Which tubes demonstrated amylase activity with a small amount of reducing sugar produced?

7 and 8

Enzymes are

_______.

made of protein

are catalysts

permanently changed by the substrate

made of protein and are catalysts

made of protein, are catalysts, and permanently changed by the substrate

enzymes are made of proteins and catalysts

The substance that the enzyme acts on is called its _____

subtrance

Amylase is secreted by

_______.

the stomach

the small intestine

the salivary glands

the pancrease

both the salivary glands and the pancreas

both salivary glands and pancreas

Maltose is a

_______.

monosaccharide

complex carbohydrate

type of starch

disaccharide

disaccharide

List the substrate and the subunit product of amylase

starch, maltose

When using the Benedict's assay, which color would indicate the most reducing sugar

present?

blue

green

reddish brown

orange

reddish brown

IKI is used to test for the presence of

_______.

protein

starch

cellulose

maltose

starch

Substrates are held in the active site by

_______.

hydrogen and ionic bonds

hydrogen and covalent bonds

covalent bonds

ionic bonds

hydrogen bonds

Check answer

hydrogen and ionic bonds

What is the difference between cellulose and

starch?

They are linked differently.

Cellulose is a polysaccharide, but starch is not.

They are in different locations in the animal cell.

Starch is a polysaccharide, but cellulose is not.

Check answer

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a

Test tube 3 did or did not show a positive Benedict's test, because amylase doesn't digest cellulose.

DID NOT

can bacteria break down cellulose?

YEs

What occurred when pepsin was

boiled?

The enzyme didn't work because the incubation temperature was too high.

It was inactivated.

The enzyme didn't work because the pH was too low.

Its activity increased.

it was inactivated

pepsin is

a. a peptidase and an enzyme that digests protein
b. an enzyme that digests protein
c. a protein that digests protein
d. a peptidase
e. a peptidase, an enzyme that digests protein and a protein that digests protein

e, a peptese, an enzyme that digest protein and a protein that digest protein e

The optimal pH for pepsin activity is _________

a. the activity was the same at a pH of 2, 7, and 9
b. 7
c. 2
d. 9

2

Pepsin is secreted by _______.

a. goblet cells
b. acinar cells
c. chief cells
d. salivary glands

chief cells

Pepsin is classified as _______.

a. an enzyme and a substrate
b. a peptidase
c. an enzyme and a peptidase
d. a substrate
e. an enzyme

an enzyme and a p

When BAPNA is hydrolyzed, it ______. (color)

it changes from colorless to yellow

Which test tube had the highest lipase activity?

a. tube 1
b. tube 2
c. tube 4
d. tube 3

a

Why is gastric lipase NOT as active as pancreatic lipase?

a. the pH of the stomach is too low
b. the pH of the stomach is too high and no bile is present in the stomach
c. the pH of the stomach is too low and no bile is present in the stomach
d. the pH of the stomach is too high
e. no bile is present in the stomach

c

The substrate of lipase is ______.

a. fatty acids
b. triglyceride
c. monoglyceride
d. bile

triglycerides

Triglycerides _________.

a. are fats
b. dissolve poorly in water
c. are digested by lipase
d. are lipids
e. all of the above are true

e

Adding bile to triglycerides _______.

a. chemically modifies the triglycerides
b. increases the surface area of the triglycerides
c. digests triglycerides into monoglyceride and two fatty aicds
d. causes them to clump

b

Which of the following does NOT secrete a lipase?

a. the salivary glands
b. the pancreas
c. the small intestine
d. the stomach

the small intestine

Prior to lipase digestion of triglycerides, the solution would be ________.

a. more acidic and less alkaline
b. more acidic
c. more basic
d. less alkaline

more basic

Explain why pancreatic lipase would be active in both the mouth and the pancreas.

Pancreatic lipase is the most active at a pH of 7.0. The mouth and pancreas have a pH of around 7.0 which is why the enzyme should be active in these two places.