bacterial physiology

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Last updated 2:33 PM on 5/19/26
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11 Terms

1
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speed of replication

  • what is it called

  • what can be interval be

  • e coli

  • m. bovis

  • generation time or doubling time

  • interval from completion of septum formation to septum formation or form bacterial cell to two cells at the same stage

  • 20 min for e coli

  • 16hrs for mycobacterium bovis

2
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endospsore

  • dormant, highly resistant body

  • survives under adverse environmental conditios

<ul><li><p>dormant, highly resistant body</p></li><li><p>survives under adverse environmental conditios</p></li><li><p></p></li></ul><p></p>
3
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what is sporulation and germination

  • sporulation- spore formation

  • germination- reverting back to vegetatie cycle (actively growing)

4
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complete culture media

grows almst all culturable bacteria eg blood agar

5
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selective cuture media

and example

selects agaisnt some bacterial species eg macconkey (gut), mannitol salt

6
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differential culture media

different appearance of bacteiral species e,g macconkey

7
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enrichment

and examples

  • enhances the growth of a particular bacterial species eg selenite broth, salt broth

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ingredients in MacConkey

  • peptone a water soluble mix of polypeptides and amino acids (pancreatic digest of gelatin)

  • proteose peptone (meat and casein)

  • lactose monohydrtae

    • fermentable carb

  • bile salts

    • selective

    • most gram positive can not grow in presence of bile salts and crstal violet

  • NaCL

  • neutral red

    • differential- pH indicator

    • red pH <6.8

    • colourless ph>6.8

  • crystal violet-

  • agar

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incubation conditions

  • temp

  • ph

  • nroamlly 37 but there are

    • tehrmophiles

    • mesophules

    • psychotrophs

    • psychrophiles

  • pH normally neutral

    • but there are acidophilic and alkophilic

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testing oxygen requirement

  • strict aerobe will only grow at the top

  • obligate anaerobe will only grow at the bottom

    • sealed jar and paladium catalyst (2H2 + O2 = 2H2O )

  • facultative anaerobe

    • growing through the entire tube

  • microaeorophil

    • reduced oxygen

    • can have a jar and a candle, the flame will burn off O2 and release CO2

<ul><li><p>strict aerobe will only grow at the top</p></li><li><p>obligate anaerobe will only grow at the bottom</p><ul><li><p>sealed jar and paladium catalyst (2H2 + O2 = 2H2O )</p></li></ul></li><li><p>facultative anaerobe</p><ul><li><p>growing through the entire tube</p></li></ul></li><li><p>microaeorophil</p><ul><li><p>reduced oxygen</p></li><li><p>can have a jar and a candle, the flame will burn off O2 and release CO2</p></li></ul></li></ul><p></p>
11
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importance of understanding commensals

  • may outcompete or regulate pathogens

  • can act as opportunistic pathgogens

  • culture can be confounded by commensal growth

<ul><li><p>may outcompete or regulate pathogens</p></li><li><p>can act as opportunistic pathgogens</p></li><li><p>culture can be confounded by commensal growth</p></li></ul><p></p>