Hematology Special Tests and Stains/Inclusions/Markers

FLAER Test Measures

CD55 and CD59GPI Anchor Proteins

FLAER Test Indications

PNH

Flow Cytometry Measures

CD55 and CD59 deficiency

Flow Cytometry Indications

PNH

Heinz Body Prep Indications

Unstable Hemoglobins and G6PD Deficiency

Sickle Cell Screen Indications

HbS

Kleihauer-Betke Acid Elution Measures

Resistance of Fetal Hemoglobin

Kleihauer-Betke Acid Elution indications

Fetal-Maternal Hemorrhage

Hemoglobin Electrophoresis Indications

Suspected Hemoglobinopathies

Cold Agglutinin Titer Indications

Cold Autoimmune Hemolytic Anemia

Donath Landsteiner Test indications

Paroxysmal Cold Hemoglobinuria

Cold Agglutinin Titer Measures

IgM Anti-I

Donath Landsteiner Test Measures

IgG Anti-P

Prussian Blue stain indicates ____ which can signify _____ anemia

Iron

Sideroblastic anemia

LAP (Leukocyte Alkaline Phosphate) significance

P.vera ; CML decrease, Leukemoid reaction increase

Paroxysmal Cold Hemoglobinuria specificity

Anti-P

Cold Agglutinin Titer Specificity

Anti-I

Peroxidase/Sudan Black indicates for _____ and is significant by being a precursor for ______

Myeloperoxidase

Myeloids

Specific Esterase indicates ____ precursor and is it negative/positive for Monocytic Leukemia

Granulocyte

Negative

Non-Specific Esterase indicates ____ precursor and it is negative/positive for Monocytic Leukemia

Monocyte

Positive

Tartrate-Resistant Acid Phosphate (TRAP)

Hairy Cell Leukemia

Auer rods

Acute Myeloid Leukemia

CD13, CD33, CD117

Myeloid/Monocytoid Leukemia

CD41, CD42, CD61

Megakaryocytic Leukemia

CD14, CD64

Monocytoid Leukemia

CD2, CD3, CD5, CD7

T-cell Neoplasms

CD10, CD19, CD22

B-cell Neoplasms

CD34 indicates for

stem cells

CD38, CD138 indicates for

Plasma Cell Markers

CD71

Erythroleukemia

CD45 is found on all:

leukocytes

CD103, CD11c, CD25

HCL

Jak 2 Mutation

PV

Chloroacetate Esterase is an example of:

A) Specific Esterase

B) Non-specific esterase

A

Alpha-Napthyl Butyrate is an example of:

A) Specific Esterase

B) Non-specific esterase

B