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Water polarity
uneven charge distribution (O = δ−, H = δ+)
Hydrogen bonds
weak attractions between water molecules (2 other h and one O)
Cohesion
attraction between water molecules
Adhesion
attraction between water and other substances
Surface tension
elastic surface caused by cohesion
Hydrogen bonds (how they form)
Hydrogen bond forms between H (δ+) of one molecule and O (δ−) of anothe
Condensation (dehydration synthesis)
Joins monomers → polymer
Releases water
Forms covalent bonds
Hydrolysis
Breaks polymers → monomers
Uses water
Peptide bond
covalent bond between amino acids
Dipeptide
2 amino acids, 1 water removed
Tripeptide
3 amino acids, 2 waters removed
Peptide bond formation removes:
OH from carboxyl group
H from amino group
Amphipathic molecules
both hydrophilic & hydrophobic regions
Phospholipid heads
polar, hydrophilic
Phospholipid tails
nonpolar, hydrophobic
Steroids (e.g. testosterone)
Lipid
Hydrophobic
Insoluble in water
Starch
Storage polysaccharide
Found in plants
Made of glucose
Easily broken down for energy
Cellulose
Structural polysaccharide
Found in plant cell walls
Provides rigidity
Structural difference
Different glucose linkages → different function
Lock-and-key model
Active site = rigid
Substrate fits exactly
Induced-fit model
Active site changes shape
Improves binding efficiency
Activation Energy
Enzymes lower activation energy
They orient substrates to make bond breaking/forming easier
Increasing substrate concentration
increased rate
Plateau occurs when:
All active sites are saturated
Enzyme becomes limiting factor
Rising temperature for enzymes
increased enzyme activity (more collisions)
Optimum temperature
peak activity
High temperature → denaturation
Bonds break
Active site loses shape
Enzymes have an optimum pH
Deviating pH alters charges
Extreme pH → denaturation
Graph interpretation:
Steepest slope = highest activity
Lower slope = reduced activity
Anabolic reactions
Build complex molecules
Require energy
Examples: photosynthesis, protein synthesis
Catabolic reactions
Break molecules down
Release energy
Examples: respiration, digestion
Ribosomes
Protein synthesis
Translate mRNA
Rough ER
Protein folding and transport
Golgi apparatus
Modifies, packages proteins
Mitochondria
ATP production
Cellular respiration
Exocrine Cells
Abundant RER → enzyme production
Large Golgi → packaging
Vesicles → exocytosis
Prokaryotes
No nucleus
Circular DNA
No membrane-bound organelles
Smaller
Eukaryotes
-Nucleus present
Linear DNA
Membrane-bound organelles
Light microscope
Uses visible light
Lower resolution
2D images
SEM
Uses electrons
3D surface images
High resolution
Why electron microscopes show more detail
Shorter wavelength
Less diffraction
Atypical Cells
Multinucleated muscle cells
Anucleate red blood cells
Fungi with shared cytoplasm
Cell Specialization
Bryophyte cells
Cell wall
Chloroplasts
Large vacuole
Red blood cells
Biconcave shape
No nucleus
Maximizes oxygen transport
Stem Cells
Undifferentiated cells
Can become specialized
Important for:
Growth
Repair
Regeneration
Gene Expression
Turning specific genes on/off
All cells have same DNA, different expression
Phospholipid bilayer:
Hydrophilic heads outward
Hydrophobic tails inward
Embedded proteins:
Channels
Carriers
Receptors
Simple diffusion
Passive
Small nonpolar molecules
Facilitated diffusion
Passive
Channel or carrier proteins
Active transport
Requires ATP
Against concentration gradient
Osmosis
Movement of water
From high → low water concentration
Through semipermeable membrane
Hypotonic
Water enters cell
Cell swells
Hypertonic
Water leaves cell
Cell shrinks
Isotonic
No net movement
Plant vs Animal in Hypertonic Solutions
Animal cell → shrinks
Plant cell
Membrane pulls from wall
Plasmolysis
Cell wall remains rigid
Why Active Transport Needs Energy
Moves substances against gradient
Requires ATP
Paramecium
Food vacuoles → digestion
Contractile vacuole → water balance
Specialized organelles maintain life
Contractile Vacuole
Pumps excess water out
Prevents bursting
Critical in hypotonic environments
Compartmentalization
Increases efficiency
Allows:
Optimal conditions
Separation of reactions
Greater control
Vesicle Transport
Proteins made in RER
Modified in Golgi
Transported in vesicles
Released via exocytosis
Membrane Fluidity
Higher temperature → more fluid
Unsaturated tails → more fluid
Saturated tails → less fluid
Cholesterol
Prevents membrane from becoming too rigid or too fluid
High SA:V
faster diffusion
Surface Area : Volume Ratio
Small cells more efficient
Large organisms compensate with:
Membrane folds (microvilli)
Transport systems
Specialized shapes