chemiosmosis theory

chemiosmosis

the ability of a cell to use proton notice force to do work. the “work” performed is changing of ADP to ATP through phosphorylation using ATP synthase

chemiosmotic theory

electron transport has 2 roles: transport electrons down the chain to form water, and pump hydrogen ions out to build proton motive force and make ATP by chemiosmosis

came up with chemiosmosis theory

Peter Mitchell

evidence for chemiosmotic theory

pH is higher in the matrix than the inner membrane - supporting idea of H+ being pumped out (high pH means low H+). also noticed some things when mitochondria was exposed to detergent and dinitrophenol respectively

mitochondria exposed to detergent

no ATP was made but water was. detergent destroyed the lipid bilayer and a gradient could not be established

exposed mitochondria to dinitrophenol

made water but no ATP, causes the uncoupling of oxidative phosphorylation, producing heat rather than ATP