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Traits
Structure Impacts Function
Amino Acid Patterns Determine Structure
Core - Hydrophobic (Nonpolar)
Surface - Hydrophilic (Polar)
Amino Acid Structure
COOH, H, H2N, R
Electronegativity Difference and Polarity
O, N, - Polar
O-H - Non Polar
C - H - Non Polar
Six Polar Neutral Amino Acids
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Ser, Thr, Cys, Asn (Y), Gln (Q), Tyr (Y)

Phosphorylation
Targets the -OH
Kinase = Phosphorylation with ATP
Phosphorylation changes structure by adding negative to the surface. In return changing function
Phosphomnetic Effect
SYT
Ser, Tyr, Thr

Disulfide Bonds (THIOLS)
SDS-PAGE
Only form from 2 Cys residues
Reduction breaks the bridge (Intra/Inter)

Asp (N) and Glu (Q): AMIDES
Glu has extra carbon
Non Polar (C-H) (C-C)
Aliphatic Amino Acid
Non Aromatic, Hydrocarbon Tails
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Branched Amino Acids
Leucinezc Isolleucine, Valine
Aromatic Aminos
Phenylalaninine, Tyr, W
My wife’s aroma.

Acidic Amino Acids
Neg Charge, due to (COO-)
Aspartate (D) and Glutamate (E)
Asp, Glu
At pH 7.4, pka is 4.
DE

Basic Side Chains
Carry positive charge
Lysine (H) , Arginine (R), Histidine (H)
KRH (Knights Riding Horses)
10, 12.5, 6 = pka
H is only partly pronated in the bottom/interior

Heterocycles
Imidazole (2N) sits on histidine
Indole (1N) on tryptophan
NOT:
Benzene (Phe)
Phenol (Tyr)
Henderson - Hasselbalch
pH = pKa + log ([A-]/[HA])
PH = A / The Environment
PKA = HA / The Molecule

Typical pKa values
Cysteine - 8
Tyrosine - 10
Carboxylic acids get charge when deprotonated .
Acidity of the whole molecule
Isoelectric point = where the charge is zero (zwitter ion)
PI represents all molecules
pi>ph (molecule wins)
ph>pi (env wins)
How to calculate the pI
Average of pKa = pI
For an amino acid: avg the two lower pKa’s for an acidic amino acid, and the two higher pkas for a basic amino acid.
carboxylic acid ph = 2
amino acid ph = 9
side chain ph depends on R
increasing pI = more basic

Isoelectric focusing: separate by pI
anode +
cathode -