Question 4: Contrast of Global vs. Site-Specific Epigenetic Analysis Dr. Yan Yan's research on nanoscale shape utilized both bottom-up mass spectrometry and ChIP-seq to investigate histone modifications. Task: Contrast the information provided by these two tools. Explain why mass spectrometry was sufficient to identify the global decrease of H3K27me3 in response to spiky nanoparticles (GNP2), but why ChIP-seq was necessary to link these changes to the "mechanical sensing" of the cell and the reprogramming of specific synapse-related gene pathways.
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