Lab 2 Culturing Bacteria

These flashcards cover important terms and definitions related to culturing bacteria, types of culture media, and laboratory practices.

Culture Media

Substances used to support the growth of microorganisms.

Aseptic Technique

Methods used to prevent contamination from pathogens.

Pure Culture

Culture containing a single species of microorganism.

Mixed Culture

Culture containing multiple species of microorganisms.

Nutrient Agar

General-purpose medium used for growing a wide range of microbes.

Selective Media

Media designed to inhibit the growth of certain microbes while allowing others to grow.

Differential Media

Media that displays visible differences in the growth characteristics of different organisms.

Quadrant Streak Plate

Method used to isolate pure cultures by diluting microbial samples across an agar surface.

Enriched Media

Media that contains additional growth factors to support fastidious microbes.

Reducing Media

Media designed to remove oxygen to support the growth of anaerobes.

Autoclave

Device used to sterilize culture media by applying high pressure and temperature.

Citrate Agar

A selective and differential medium used to test the utilization of citrate.

Tryptic Soy Broth (TSB)

A type of liquid nutrient media used for growing a wide range of bacteria.

Nutrient Broth (NB)

A liquid media used to support the growth of non-fussy bacteria.

Hemolysis

The breakdown of red blood cells, often tested in enriched differential media like blood agar.

Obligate Aerobe

Microorganism that requires oxygen for growth.

Facultative Anaerobe

Microorganism that can grow with or without oxygen.

Obligate Anaerobe

Microorganism that cannot grow in the presence of oxygen.

Capnophiles

Microorganisms that require increased levels of carbon dioxide for growth.