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[How were the two forms of chromatin originally distinguished?]
Cytologically by how intensely they stained – euchromatin stains less intensely (decondensed), while heterochromatin stains intensely (condensed/tighter packing).
[What is the Barr body and how does it relate to heterochromatin?]
The Barr body is an example of heterochromatin that stains intensely due to tighter packing (inactivated X chromosome).
[What are the different scales of 3D genome organisation?]
Nucleosome = ~147bp of DNA, Enhancer loops can span >100Mb, TADs are megabase-scale, Chromosome territories are the largest scale.
[What technique was used in the Bolzer et al., 2005 study shown on this slide?]
Chromosome painting – a technique used to visualise chromosome territories in the nucleus.