Clinical Chemistry 2 - Laboratory - 5 - Lactate Dehydrogenase - Complete, lacking info doesn't affect anything

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Last updated 1:58 PM on 2/25/26
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45 Terms

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Non-specific

LDH is specific or non-specific?

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Oxidoreductase

LDH classification

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1.1.1.27

LDH enzyme commission number

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Lactic acid, Pyruvic acid

LDH catalyzes the interconversion of these 2 acids

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Zinc

Element found in LDH; part of glycolytic pathway

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4

LDH amount of subunits

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H, M

2 possible forms of LDH subunits

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Nicotinamide adenine dinucleotide

LDH coenzyme

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Heart, RBC, Kidneys, Lungs, Pancreas, Spleen, Skeletal muscle, Liver, Intestine

LDH tissue sources

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Pernicious anemia, Hemolytic anemia, Acute myocardial infarction, Hepatic carcinoma, Toxic hepatitis

LDH increased in these conditions

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LDH flipped pattern

Why is LDH important in AMI?

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LD2 > LD1

Normal LD pattern

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LD1 > LD2

LDH flipped pattern

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Wrobleuski-La Due method

LDH determination method used in laboratory activity

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LDH Reverse Reaction

Principle of method

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pH 7.4-7.9

Wrobleuski La Due method pH

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Forward/Direct Reaction

What kind of reaction is seen in Wacker method?

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pH 8.8

Wacker method pH

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340 nm

Wacker method wavelength

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Wacker method

Most commonly used method for LDH (produces a positive rate)

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pH 7.2

Wrobleuski La Due method pH

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Wrobleuski-La Due method

What is faster? Wrobleuski La Due or Wacker method?

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Non-hemolytic serum

Preferred specimen

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Heparinized or EDTA plasma

Other acceptable specimen

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15-25°C

LDH specimen temp if stable for 24 hours

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2-8°C

LDH specimen temp if stable for 3 days

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20°C

LDH specimen temp if stable for 6 weeks

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225-450 U/L

LDH expected value

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0.017

LDH U/L to ukat/L conversion factor

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43.8-1200 U/L

LDH kit measuring range

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2 mL

LDH working reagent volume

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0.040 mL

LDH sample volume

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1 minute, 37°C

LDH incubation time and temperature

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Bilirubin up to 20 mg/dL, Triglycerides up to 500 mg/dL, Hemoglobin up to 5 g/dL

LDH interferences

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340 nm

LDH absorbance

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40 uL

LDH sample volume in uL

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2000 uL

LDH reagent volume in uL

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60 seconds

LDH lag time

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60 seconds

LDH kinetic interval

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8095

LDH kinetic factor

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4

LDH number of readings

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37°C

LDH reaction temperature

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Water

LDH blank with?

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U/L

LDH unit of measurement

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Decreasing

LDH reaction direction