Aseptic Technique and Streaking Plates

Flashcards about Aseptic Technique and Streaking Plates

Sterilization

To render something void of life; in other words, the removal of all microorganisms and other pathogens from an object or surface by treating it with chemicals or subjecting it to high heat or radiation.

Inoculating loops

Pieces of wire with a circle at the end; sterilized immediately before use in a Bunsen burner or microincinerator.

Autoclaves

Used to sterilize equipment and supplies by subjecting them to high pressure with steam at 121°C (249°F) for 15–20 minutes.

Media Growth medium or culture medium

Solid or liquid designed to support the growth of microorganisms or cells.

Inoculation

The act of introducing microorganisms into/onto culture media.

Streaking for isolation

A method used to isolate individual bacterial colonies in order to obtain a pure culture.