microbio lab Exercise 3
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25 Terms
MAC plate Escherichia coli
GROWTH: yes
APPEARANCE: purple
MAC plate Proteus mirabilis
GROWTH: yes
APPEARANCE: clear
MAC plate Staphylococcus aureus
GROWTH: no
APPEARANCE: N/A
MSA plate Staphylococcus aureus
GROWTH: yes
FERMENTATION: yes (yellow)
MSA plate Staphylococcus epidermidis
GROWTH: yes
FERMENTATION: no (clear)
MSA plate Proteus mirabilis
GROWTH: none
FERMENTATION: N/A
CNA plate Staphylococcus aureus
GROWTH: beta hemolytic
GRAM STAIN: +
CNA plate Escherichia coli
GROWTH: no
GRAM STAIN: -
MH plate Pseudomonas aeruginosa
APPEARANCE: medium, green
GRAM STAIN: -
MH plate Escherichia coli
APPEARANCE: medium, creamy
GRAM STAIN: -
MH plate Staphylococcus aureus
APPEARANCE: medium, creamy
GRAM STAIN: +
BAP plate Pseudomonas aeruginosa
GROWTH: beta hemolytic
DESCRIPTION: shiny, large, flat
BAP plate Escherichia coli
GROWTH: beta hemolytic
DESCRIPTION: medium/large, grey
BAP plate Staphylococcus aureus
GROWTH: beta hemolytic
DESCRIPTION: medium, creamy
BAP plate Staphylococcus epidermis
GROWTH: non-hemolytic
DESCRIPTION: small, white, opaque
BAP plate Proteus mirabilis
GROWTH: non-hemolytic
DESCRIPTION: swarms, smelly (like Elijah)
BAP plate Streptococcus pneumoniae
GROWTH: alpha hemolytic
DESCRIPTION: medium, dipped in, clear
mannitol salt agar plate (MSA)
SELECTIVE: yes (7.5% NaCl)
DIFFERENTIAL: yes (1% mannitol)
DESCRIPTION: ferment = yellow, non-fermenter = clear
sheep blood agar plate (BAP)
SELECTIVE: no
DIFFERENTIAL: yes (5% RBCs)
DESCRIPTION: demonstrates hemolysis (beta/alpha/non)
plain Mueller Hinton plate (MH)
SELECTIVE: no
DIFFERENTIAL: no
DESCRIPTION: N/A
MacConkey agar plate (MAC)
SELECTIVE: yes (bile salts + crystal violet)
DIFFERENTIAL: yes (lactose);
DESCRIPTION: selects for gram-negative (-)
fermenter = (+)/purple, non-fermenter = (-)/clear
Columbia Colistin Nalidixic Acid agar plate (CNA)
SELECTIVE: yes; Columbia agar base (nutrient w/ peptides), 5% sheep blood, colistin and nalidixic acid (antimicrobial agents)
DIFFERENTIAL: yes; (5% sheep RBCs for hemolysis), high carbs
DESCRIPTION: alpha/beta/non-hemolytic, selects G+, suppresses G-
alpha hemolysis
partial RBC clearing (left)
beta hemolysis
complete RBC clearing (middle)
non hemolysis
no clearing of RBCs (right)
