Mass Spectrometry Lecture

These flashcards cover key concepts and definitions related to mass spectrometry and proteomics, as outlined in the provided lecture notes.

What are molecular ions produced by Electron Impact in mass spectrometry?

Radical cations M+.

What does MALDI produce in terms of charged particles?

Singly charged cations and anions, such as [M+H]+ and [M-H]-.

What type of ions does Electrospray produce?

Multiply charged cations and anions, e.g., [M+nH]n+.

What happens to ions in the first analyser of MS/MS experiments?

Ions produced are selected and then collisionally activated by a collision gas.

What are the applications of Triple Quadrupole instruments in mass spectrometry?

Popular for quantitative proteomics due to robustness, reliability, and low cost.

Who designed the Q-TOF mass spectrometer?

Professor Howard Morris at Imperial College in the mid 1990s.

What is the main advantage of using multiply charged ions in MS/MS Peptide Sequencing?

They require less collisional energy than singly charged ions.

What is the significance of trypsin in peptide sequencing?

Tryptic peptides have a minimum of two charges, aiding in identification.

What is the predicted m/z of a peptide if [M+H]+ = 1001?

It indicates a molecular mass of 1000 Da.

What is one reason why shotgun proteomics is necessary?

It allows analysis of all proteins in a system, especially low abundance and membrane proteins.