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Origin of dentin
formed by odontoblasts that differentiated from ectomesenchymal cells of the dental papilla
Dental papilla is the formative organ of dentin and eventually becomes the pulp
Composition of dentin
Inorganic component - 87% hydroxyapatite crystals, carbonate apatite and fluroapatite
Organic component - 13% collagen fibres and small amounts of other proteins (dentin phosphoprotein, dentin matrix protein 1, dentin sialoprotein and other) and water
when does dentinogenesis begin
after the final differentiation of the odontoblasts and subodontoblasts
Stage 1 (of formation of dentin matrix and mineralisation) - formation of organic matrix
Type 1 collage is key structural component in matrix of dentin
First sign of dentin formation is appearance of Korff’s fibres, they are large diameter collagen fibrils, they originate deep among the odontoblasts and extend towards the inner epithelium
Odontoblasts differentiate in the pre-existing ground substance of dental papilla, the first dentinal collagen synthesised by them is deposited in this ground substance
Stage 2 (of formation of dentin matrix and mineralisation) - mineralisation
Phase first appears within matrix vesicles as single crystals
Crystals grow rapidly and rupture from the confines of the vesicle to spread as a cluster of crystallites that fuse with adjacent crystals and form a continuous layer of mineralised matrix
Globular mineralisation - involves deposition of crystals in several discrete areas of matrix, with continuous crystal enlargement and fusion eventually a single calcified mass is formed
Linear mineralisation - in slower depositions of crystals, mineralisation front looks like general and mineralisation is linear
what is globular mineralisation?
involves deposition of crystals in several discrete areas of matrix, with continuous crystal enlargement and fusion eventually a single calcified mass is formed
what is linear mineralisation?
in slower depositions of crystals, mineralisation front looks like general and mineralisation is linear