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Acronym for order of draw
Big Lions Run Greatly Growling Loudly Galloping
Order of draw?
Blood culture
light blue
Red
Green/Gold
Lavender
Gray
Yellow (SPS)/ Blood culture bottle
Additive is sodium polyanethol sulfonate
Type: anticoagulant (prevent change of blood) preservative
Can be preserved in tube for 2-4 hours but then back to blood culture bottle
Must be full
8-10 inversions
Tests: AFB culture- checks for mycobacterium
Fungal and blood cultures tests too
Light blue
Additive: sodium citrate
Type: reversible anticoagulant
Used for: coagulation testing, plasma. Binds calcium to prevent clotting
3-4 inversions
Tests: PT/INR-evaluates extrinsic clotting pathway-used for warfarin monitoring
PTT/aPTT-intrinsic pathway-heparin monitoring
D-dimer-detects clot breakdown and used to rule out DVT/PE
Fibrinogen- measures fibrinogen levels, low levels indicate bleeding risk

Red
Additive: If GLASS-none. if PLASTIC-clot activator
Purpose: Produce serum
5 inversions for plastic
Tests: Monitors therapeutic drug concentrations
Antibody titers: immunity or autoimmunity activity
Hormone levels- endocrine
serum chemistry: basic metabolic tests

Gold/Tiger top
Serum seperator-clot activator and gel
Tests: electrolyte tests, kidney, liver, glucose
BMP: basic electrolytes and kidney function
Lipid panel-cholesterol
Liver panel: AST, ALT, bilirubin
Thyroid tests: TSH, T3,T4
5 inversions

Green
Lithium (light green) or sodium (dark green) heparin
Note: Lithium heparin can’t test for lithium levels
8-10 inversion
STAT chemistry- quick tests
Troponin tests, ammonia-elevated in liver failure, electrolytes, lactate-evaluates tissue hypoxia(shock)

Lavender/Purple
EDTA(K+ based)
8-10 inversions
Tests: CBC- tests WBC RBC and platelets
A1C-3 month average blood glucose
ESR-checks for inflammation
Reticulolyte- measures immature RBC
Blood bank testing-pink EDTA

Gray
Sodium Fluoride (preserves glucose)/potassium oxalate (anticoagulant
Tests for Glucose and lactic acid and alcohol level

Serum vs plasma
Plasma is before clotting and serum is after clotting
Plasma before, serum after