Lab 5

Defined Synthetic Media

growth media which the exact chemical composition and quantities are known

Complex Media

the exact chemical composition is unknown due to the addition of yeast, plant or animal products

What is the physical differences of media

presence or absence of agar

Liquid media

broths that have been dissolved in water and dont contain agar

Semi-solid media

contains nutrients to meet the nutritional requirements of the microbe and a small percentage of agar

Solid media

similar to semi solid but contains more agar

Selective media

contains ingredients to act to encourage the grow of some types of bacteria while inhibiting the growth of other types of bacteria

Differential media

contains ingredients that cause distinct types of bacteria to grow differently on the same media

Enriched media

used to grow fastidious microorganisms requiring additional nutrients and growth factors

Anaerobic media

specialized type of media that are used to grow bacteria that require an absence oxygen

Transport media

contains ingredients that serve to maintain the microorganism being transported without permitting its multiplication

Subculturing

moving bacteria from a media source in which they have been growing to a new sterile media source

Nonpigmented

cream, tan, white

Pigmented

purple, red, yellow

Bacterial colony

A visible mass of bacteria on a solid culture medium

Pellicle

Thin film formed on broth culture surfaces

Facultative anaerobe

Organism that makes ATP through aerobic respiration

6 specific margin characteristics

Entire, undulate, lobate, filamentous, rhizoid, serrate

What factors affect the formulation of culture medium

Nutrition, pH, osmotic pressure