micro lab TEST NOW pt.3
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27 Terms
what’s this?
kirby-bauer antibiotic test
what are we comparing?
the sensitivity and resistance of gram positive and gram negative
no growth around disc mean?
sensitive
to determine the effectivness of antibiotic on growth we?
measure the inhibition in mm
if there’s regrowth in zone of inhibition it means?
bacteria had preexisting mutation for resistance
greater diameter
greater effectiveness
no clearing
bacteria resistant
what is this?
disinfectant and antiseptic test
what are we comparing?
sensitivity and resistance of gram positive and gram negative bacteria
no growth around disc means
sensitive
growth around disc is?
resistant
what do we measure?-clearing
zone of inhibition around disc
what’s this?
uv light box
what bacteria is this?
serratia marcescens
what is this called?
uv radiation
how many nm?what wavelenght of uv light was used in our expirement?
254 nm
what form of mutation is caused by exposure to uv light
thymine dimers
if theres no growth it means?
sensitive to uv
uv must directly … the bacteria to kill bacteria
contact
3 factors determine effectiveness of uv treatments
time of exposure, amount of shielding, distance from bacteria
what bacteria is this?
bacillus
what does bacillus make?
endospores
when does uv exposure effectively kill bacteria ?
uv must directly contact bacteria to kill bacteria
name the 2 variables that we tested that influence the effectivness of uv exposure
uv exposure and amount of shielding
why is bacillus more resistance to uv exposure than serratia?
make endospores
why does the plate that was irradiated 2 minutes with the lid on grow normally?
lid blocked the UV light .
uv light can have?
mutation repair-dna repair