micro lab quest 2

What is the name of the microscope we use in lab?

Compound light microscope

How is magnification achieved using a microscope?

Magnification is achieved through two lens system that bends light rays to make them appear larger

Define ocular lens

The lenses that you look through and they provide 10x magnification. They can be adjusted for the distance between your eyes.

Define Objective lens

This is the 4x, 10x, 40x, and the 100x lens

4x is called"?

Scanning power

This is the lens furthest from the slide

40x total magnification

It has the largest field of view and the smallest image

10x is called?

Low power

Lens medium distance to the slide

100x total magnification

It has an intermediate field of view and an intermediate size image

40x is called?

High power

Lens closed to the slide

400x total magnification

This has the smallest field of view and the largest image

100x is called?

Oil immersion

Lens touches oil on slide

1000x magnification

Only used with oil on lens

Define parfocal

Once an object is in focus at low power, it will remain coarse focus when you switch to the higher power objective lens

Define resolution

Clarity of an image and is determined by magnification and the amount of light passing through the condenser

Define field of view

Total amount of specimen that is visible, will decrease any time you magnify an object

What is the importance of the condenser

Lens that forms a cone of light that illuminates the specimen and adjusts contrast

What’s the importance of the iris diaphragm

Controls the amount of light focused on a specimen. This must be changed every time you change magnification

What is the importance of oil immersion

This is used to decrease refraction of light rays by preventing them from coming into contact with the air in between the slide and 100x objective lens