Prokaryote Biology Exam Four - Uark Lessner

A transcriptional regulatory protein that functions to postively regulate transcription is called a(n)

activator

Where would you typically find the sequence that a regulatory protein binds to (i.e. has an affinity for)?

adjacent/near to the promoter(s) of a gene(s)

Which of the following would most likely bind to mRNA to prevent translation?

sRNA

Bacillus subtillis increases in the intracellular concentration of __________ and calcium during sporulation to stabilize DNA in the mature DNA in the mature endospore.

dipicolinic acid

E. Coli uses ArcB to sense the redox state of ____________ as a mechanism to indirectly sense the levels of oxygen.

quinones

Cells of B. subtillis carry out __________ primarily to delay sporulation

cannibalism

A mutation in the promoter sequence upstream of a tRNA gene would most likely affect

transcription of the tRNA gene

Which of the following is incorrect about a composite transposon?

does NOT require transcription and translation to move locations

Which of the following is NOT involved in the process of cannibalism by B. subtillis

sigma F

Bdellovibrio bacteriovorus preys on other Gram negative bacteria, such as E. coli, by specifically invading the ___________ which is present in the cell envelope of Gram negative bacteria.

periplasm

Which of the following is INCORRECT about the differences between vegetative cells and endospores of B. Subtillis?

spores contain a higher internal concentration of water than vegetative cells

Catabolite repression in E. Coli involves all of the following EXCEPT

allolactose

Expression of the ent genes, encoding enterochelin (siderophore) biosynthesis enzymes, would be _____________ in a fur deletion mutant of E. coli when compared to wild type when both strains are grown under low iron conditions

the same

Caulobacter bacteria are unique because they divide by asymmetrical cell division, where a ___________ cell must first differentiate into a stalked cell before division can occur.

swammer

Which of the following is NOT a DNA-binding protein?

LacZ

Which of the following mutations would most likely NOT result in a frameshift within a structural gene?

transversion

Which of the following is INCORRECT about the methyl-mismatch DNA repair system?

it is an error-prone system

Which of the following is INCORRECT about rhyB, the sRNA involved in the Fur regulon in E. coli

is a product of translation

Which of the following would specifically recognize and bind to an operator?

Fur

In bacterial two-component regulatory systems, which of the following would typically be a DNA-binding protein?

response regulator

Which of the following is NOT a known effector molecule in E. Coli?

SpoIIAB

Which of the following is NOT a known mutagen to bacteria

N2

Which of the following is INCORRECT about transposition?

allows DNA to directly move from one bacterial cell to another bacterial cell

Which of the following could NOT contain a transposon

all - virus genome

3 multiple choice options

Which of the following is incorrect about bacterial biofilms?

requires production of lipid matrix to encase cells

To activate the SOS repair system in E. Coli, _____________ senses single-strand DNA and inactivates LexA, which results in induction of the genes encoding DNA polymerase V, the translession polymerase.

RecA

Which of the following conditions would E. Coli most likely exhibit diaxuic growth

when given glucose and lactose

The heat shock response in E. Coli directly involves all of the following EXCEPT?

sigma70

In negative regulation, when the inducer is present within a bacterial cell then

the cognant repressor does not bind DNA

Which of the following is NOT involved in regulating sigmaF in B. subtillis?

sigmaE

Which of the following is NOT involved in quorum sensing by bacteria?

sigma32 (RpoH)

In negative regulation, when the co-repressor is present within a bacterial cell then

the cognant repressor binds DNA

Which of the following is INCORRECT about operators?

they are directly involved in regulating translation

Deletion of rpoH (sigma 32) in E. coli would most likely affect the ability of E. coli to survive

elevated temperature

A increase in the concentration of ppGpp in E. coli would most likley result in a decrease in the transcription of ____________

rRNA gene

Which of the following would most likely be the outcome of a point mutation in a ribosome-binding sequence?

altered translation of the associated gene(s)

A mutation in the gene encoding adenylate cyclase would most likely affect the ability of E. Coli to respond to changes in ______________

glucose levels

Use the following (lowest, medium, highest) to describe the level of transcription of the lac operon that is expected when the specified E. coli cells are provided the indicated carbon source(s)

wild-type e. coli provided glucose only

lowest

Use the following (lowest, medium, highest) to describe the level of transcription of the lac operon that is expected when the specified E. coli cells are provided the indicated carbon source(s)

wild type e. coli provided succinate only (succinate is an non-sugar carbon source)

lowest

Use the following (lowest, medium, highest) to describe the level of transcription of the lac operon that is expected when the specified E. coli cells are provided the indicated carbon source(s)

e coli deleted of lacI provided lactose only

highest

Use the following (lowest, medium, highest) to describe the level of transcription of the lac operon that is expected when the specified E. coli cells are provided the indicated carbon source(s)

e coli deleted of LacI provided glucose only

medium

Use the following (lowest, medium, highest) to describe the level of transcription of the lac operon that is expected when the specified E. coli cells are provided the indicated carbon source(s)

e coli deleted of CRP provided glucose

lowest

Use the following (lowest, medium, highest) to describe the level of transcription of the lac operon that is expected when the specified E. coli cells are provided the indicated carbon source(s)

e coli deleted of LacY provided lactose

lowest

Bacterial cells communicate with each other primarily using different vibrational soundwaves

false

hospitals use autoclaves to sterilize tools using heat. autoclaves heat to 121 C because that is the temperature needed to kill pathogenic hyperthermophilic bacteria

false

negative regulation of transcription involves both repressors and activators

false

The deletion of one base pair (bp) in the coding sequence of a structural gene in E. Coli will always result in a change in the amino acid sequence of the encoded protein

true

Some archaeal viruses produce protein-based pyramids in the membrane of the host cell that allows gene egress (exit) of the virus from the cell

true

a nonsense mutation in the beginning of the gene encoding the catalytic subunit of RNA polymerase would most likely be a conditionally lethal mutation to e. coli

false

A repressor and an activator may regulate the expression of the same gene in E. coli

true

transposition allows a piece of DNA to move from one location to another without the use (help) of proteins (i.e. protein(s) are not required for transposition)

false

The ames test uses a mutant of salmonella to determine if a chemical is a mutagen

true

Vibrio fischeri produces light for the Hawaiian bobtail squid to allow the squid to find food at night

false

Does orfR likely play a role in regulating transcription of a cat in response to H2O2?

YES

Which of the following best describes the regulatory function of OrfR?

Repressor

Does IR1 likely play a role in regulating transcription of cat in response to H2O2

NO

Which of the following best describes the regulatory function of IR1?

Unknown, results do not support a clear function

Does IR2 likely play a role in regulating transcription of cat in response to H2O2?

YES

Which of the following best describes the regulatory function of IR2?

Operator

Which of the following best describes the function of H2O2 in regulating the transcription of cat?

inducer

Based on the results, which of the following likely describes the cat promoter?

strong

Based on the results, what mode of regulation likely controls cat transcription?

negative

You want to make a solution of 1M NaCl (MW = 58.44 g/mol) in a total volume of 1000 mL. How many grams of NaCl should you use?

58.44 g

What is the name of the regulatory protein that controls transcription of the operons encoding V-nitrogenase and Fe-nitrogenase in Methanosarcina acetivorans?

ModE