Microbiology and Integrated Infections: Microscopes

A set of fill-in-the-blank flashcards covering the components, types, and specifications of light and electron microscopes as described in Chapter 6.

The __________ lies below the stage of a light microscope.

Condenser

When the condenser is changed, the result is a __________ microscope that uses reflected light.

Dark field

The standard light microscope utilizes __________ light.

transmitted

The fixative used for samples in light microscopy is __________.

$10\%\,NBF$ (formalin)

In light microscopy, the specimen is mounted on a __________ slide.

Glass

The resolution of a light microscope is __________.

$$0.2\,\mu m$$

The objective lens identified as the scanner has a magnification of __________.

$$4\times$$

The objective lens identified as high power has a magnification of __________.

$$40\times$$

The maximum magnification for a light microscope is calculated as $10 \times 100$, totaling __________.

$$1,000\times$$

The environment within an Electron Microscope (EM) is a __________.

Vacuum

The specific fixative used for electron microscopy is __________.

$$2.5\%\,\text{Glutaraldehyde}$$

The resolution of an electron microscope is __________.

$$0.2\,nm$$

Instead of glass, an electron microscope uses a __________ slide.

Copper

To view internal structures, the __________ type of electron microscope is used.

Transmission

To produce a 3D image, the __________ type of electron microscope is used.

Scanning