Indirect Counting of Bacteria and the Effects of Temperature on Bacterial Growth - Lab Practical 4

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Last updated 6:27 PM on 6/23/26
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11 Terms

1
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For the bacteria Serratia marcescens

  • Know what pigment it makes and be able to identify it on a slant culture

  • Know which temperature it produces this pigment

  • Be able to differentiate between a slant grown at 25C and 37C

2
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Know what pigment it makes and be able to identify it on a slant culture

  • Prodigiosin (red pigment)

3
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Know which temperature it produces this pigment

  • 25C

4
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In what temperature range does psychrophile grow at?

  • -15C-20C

5
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In what temperature range does mesophile grow at?

  • 20C-50C`

6
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In what temperature range does thermophile grow at?

  • 45C-80C

7
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In what temperature range does hyperthermophile grow at?

  • 67C-105C

8
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Is using a spectrophotometer a direct or indirect method to count bacteria? What is measured?

  • Indirect method

  • Optical density`

9
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Be able to interpret a graph similar to the on in the PPT. For each growth curve:

  • Be able to determine the optimal growth temperature

  • Know which curve would be Escherichia coli and which is Serratia marcescens

  • Know how the curve would be classified: psychrophile, mesophile, thermophile, or hyperthermophile

10
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What is the optimal growth temperature of Escherichia coli?

  • 37C

11
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What is the optimal growth temperature of Serratia marcescens?

  • 25C