Spectrophotometer

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Last updated 4:50 AM on 4/30/26
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7 Terms

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What does it measure?

Absorbance (how much light passes through a solution)

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Wavelength

Color of light — set to lambda max (peak absorbance for your pigment)

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Lambda max

The wavelength where your sample absorbs the MOST light

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Calibration (blank)

Use water or buffer — this is your "zero" (0 absorbance)

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Cuvette

Small glass/plastic tube that holds the sample

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Absorbance vs concentration

Higher concentration = higher absorbance (direct relationship)

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Standard curve

Known concentrations → plot absorbance → find unknowns