Carb, Amyloid, Nucleic acid Staining Photos EX 2

PAS stain (kidney)

PAS (intestine)

PAS weak staining

PAS overstained w/Schiffs

PAS weak counterstain

PAS w/ and w/o digestion of liver

PAS w/ and w/o digestion cervix

Best Carmine stain

Mucicarmine w/Weigerts hematoxylin

Mucicarmine with Harris hematoxylin

Alcian blue 2.5

AB 2.5 and 1.0

AB overstained

Weak AB

Which is which?

A. w/o hyaluronidase

B. w/hyaluronidase

• Pink = neutral polysaccharides

• Purple = mix of neutral and acidic

• Blue = acidic only

AB-PAS

AB-PAS on cervix

Less specific than AB but more sensitive

Colloidal Iron w/nuclear fast red counterstain

Less specific than AB but more sensitive

Colloidal Iron w/van gieson counterstain

Colloidal iron + van gieson (intestine)

High Iron Diamine

Congo red

Crystal/methyl violet

Thioflavine T