Carb, Amyloid, Nucleic acid Staining Photos EX 2

PAS stain (kidney)

Basically all carbs (Neutral polysaccharides, GaGs, glycoproteins/mucins)

PAS (intestine)

Basically all carbs (Neutral polysaccharides, GaGs, glycoproteins/mucins)

PAS weak staining

PAS overstained w/Schiffs

PAS weak counterstain

PAS w/ and w/o digestion of liver

Diastase/alpha amylase digest glycogen (Type II alpha amylase is better)

PAS w/ and w/o digestion cervix

Diastase/alpha amylase digest glycogen (Type II alpha amylase is better)

Best Carmine stain

Glycogen

Mucicarmine w/Weigerts hematoxylin

Acidic glycoproteins

Mucicarmine with Harris hematoxylin

Acidic glycoproteins

Alcian blue 2.5

GaGs and acidic glycoproteins

AB 2.5 and 1.0

** AB 1.0 only dyes sulfated GaGs and sulfomucins

AB overstained

Weak AB

Which is which?

A. AB w/o hyaluronidase

B. AB w/hyaluronidase

Differentiates GaGs (digested) and acidic glycoproteins

• Pink = neutral polysaccharides

• Purple = mix of neutral and acidic

• Blue = acidic only

Differentiates between neutral and acidic glycoproteins

AB-PAS

AB-PAS on cervix

Differentiate btwn neutral and acidic glycoproteins.

Less specific than AB but more sensitive

• GaGs

• All Acidic glycoproteins

Colloidal Iron w/nuclear fast red counterstain

Less specific than AB but more sensitive

• GaGs

• All Acidic glycoproteins

Colloidal Iron w/van gieson counterstain

Colloidal iron + van gieson (intestine)

• GaGs

• All Acidic glycoproteins

Collagen is red/pink, muscle/cytoplasm is yellow

High Iron Diamine

Sulfomucins/sulfated glycoproteins.

If used with AB, can diff sulfo vs sialo mucins

Sulfo = black

Sialo and GaGs = blue

Congo red

Amyloid

Crystal/methyl violet

Amyloid

Thioflavine T

Amyloid but also keratin, paneth, zymogen granules