ELISA notes

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Last updated 5:57 PM on 4/29/26
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8 Terms

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What does ELISA stand for

enzyme-linked immunosorbent assay

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What is ELISA used for

when a disease is present, the body makes antibodies specific for the disease-- telling if the body has the disease itself.

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What are antibodies

Proteins made by the immune system cells that are specific to pathogens

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What are antigens

Molecules that initiates the generation of antibodies

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What color change indicate a positive results in a typical HRP-TMB based ELISA

Colorless to blue

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When does a standard curve in ELISA help determine

The concentration of the antigen

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In an ELISA, what would you use to coat the microtiter plate

Antigen

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<p>If an antigen was not present in step one, then what would happen to the antibodies in step two after the wash step</p>

If an antigen was not present in step one, then what would happen to the antibodies in step two after the wash step

The antibodies will wash away