culture additives

— for pH Balance and Osmotic Stability

Salts

amino acids are added in because

they’re used as building blocks for protein synthesis (less as an energy source)

albumin fucntion

they also coat surfaces, helps prevent cells from sticking to plastic and glass

at pH 7, the media is what colour (with ph indicator)

red (phenol red)

at pH 6.5, media is what colour?

yellow

at pH 8, media is what colour?

blue/pruple

bicarbonate is added for what

for balancing the pH

downsides of co -culture

upsides are they can remove metabolites and provide growth factors

2 additives that he has seen people using, out of all the ones that have been tested

treatment of patients with G-CSF prior to trigger reduces the incidence of

-

unruptured follicles (anovulation).

Treatment of patients with G-CSF does what 2 things for blastocyts?

Treatment of patients with G-CSF does what for I’mplnattation?

whats the other addiitve that is non essential but has made it in?

hyaluronic acid

how do they think HA is working?

it has been promoted as a material which can adhere to both the embryo and to

the endometrium.