HPLC and LC-MS 2

Different scan modes in mass spectrometry are used to improve __, enable quantitation, and provide structural information even when fragmentation is minimal.

Efficiency

The main scan modes in mass spectrometry include Product Ion Scan, __, and Multiple Reaction Monitoring.

Single Ion Recording/Monitoring (SIR or SIM)

The Product Ion Scan selects one __ ion to fragment in the collision cell.

precursor

In the Product Ion Scan, the second analyser scans all __ ions.

fragment

SIR/SIM significantly improves sensitivity, with around __ times increase over a full scan.

100

SIR/SIM is primarily used for __ of known compounds.

quantitation

Multiple Reaction Monitoring monitors a specific __ ion to a specific fragment ion.

precursor

High selectivity and sensitivity in quantitative analysis is achieved through __.

MRM (Multiple Reaction Monitoring)

Full Scan analyses all ions __, providing some structural information.

sequentially

Cation Exchange reaction example: R-A⁺ + B⁺ ⇌ R-B⁺ + __.

A⁺

In Ion Chromatography, the __ phase contains ionic groups.

stationary

is a problem in Ion Chromatography due to mobile phase containing ions.

High background conductivity

To reduce background in Ion Chromatography, a __ column is used.

suppressor

The __ ionization technique forms [M+nH]n+ ions and has minimal fragmentation.

Electrospray (ESI)