3) Investigating Enzyme Reactions

How can you investigate enzyme activity?

By testing how pH affects enzymes like amylase.

What solution is used to detect starch?

Iodine solution is used to test for starch.

At what temperature should you heat the water bath?

Heat it to 35°C.

What solutions do you mix in the experiment?

Mix amylase, buffer solution (pH), and starch.

How do you check for starch breakdown?

Use a pipette to sample the mixture every 30 seconds and drop it into iodine.

What does it mean if the iodine solution stays orange-brown

It means all the starch has been broken down.

What variable do you change to investigate pH effects?

Change the pH of the buffer solution.

How can you keep the experiment fair?

Control variables like enzyme concentration and solution volume

What is the formula to calculate the rate of reaction?

Rate of reaction = 1000 ÷ time taken (in seconds).