Lab 15 Protein Synthesis

The process of using DNA as a template to make mRNA is called?

Transcription

The process of using the codes in mRNA to make protein is called?

translation

The organelles that read the codes and join amino acids together are?

ribosomes

In prokaryotes the two stages of protein synthesis are:

transcription and translation

In eukaryotes the 3 stages of protein synthesis are?

Initiation , Elongation , Termination

A codon is a set of _____ nucleotides found on _____

3 nucleotides

mRNA

An anticodon is a set of ____ nucleotides found on _____

3 nucleotides

tRNA

The genetic code table has a list of ______

codons

The genetic code table is located on ____

mRNA

The genetic code table codes for each ____

amino acids

What is the start codon?

AUG (methionine)

What are the stop codons?

UAA

UAG

UGA

3'___|_ region | T A C T T C C G T C C G G A G A A T A C T |_signal___|___ 5'

Which region does the RNA polymerse bind to?

promoter region

3'___|_ region | T A C T T C C G T C C G G A G A A T A C T |_signal___|___ 5'

TAC —>

AUG

TTC—→>

AAG

CGT——>

GCA

CCG——>

GGC

GAG——→>

CUC

AAT——>

UUA

ACT ——>

UGA

How many codons are in the above mRNA?

7

During translation, to which end of mRNA will the ribosome bind?

5’ end

How many tRNA molecules would be needed to translate the above mRNA?

6 tRNA molecules

What is the sequence of amino acids in the polypeptide formed?

Met—Lys—Ala—-Gly—Leu—-Leu

If a protein is made of 100 amino acids,

a. How many nucleotides will the coding segment of its mRNA contain? _

-300

If a protein is made of 100 amino acids,

How many codons will the coding segment of its mRNA contain?

100

If a protein is made of 100 amino acids,

How many tRNA molecules will be needed to make the polynucleotide using this mRNA?

100