GRAM + ENDOSPORE STAIN

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Last updated 7:01 AM on 4/23/26
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9 Terms

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Gram stain purpose
differentiate bacteria by cell wall
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Gram stain steps

1. crystal violet 2. iodine 3. ethanol 4. safranin

<p>1. crystal violet 2. iodine 3. ethanol 4. safranin</p>
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Gram positive
purple, thick peptidoglycan
purple, thick peptidoglycan
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Gram negative
pink, thin wall
pink, thin wall
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Key step in gram stain
ethanol (removes dye from Gram -)
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Endospore stain steps

1. malachite green + heat 2. rinse3. safranin

<p>1. malachite green + heat 2. rinse3. safranin</p>
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Endospore result
spores = green, cells = red
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Endospore function
survival in harsh conditions
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Spore-forming bacteria
Bacillus, Clostridium