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Flashcards covering cell structures, functions, organelle details, and microscopy based on the provided biology lecture notes.
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Resolving Power (Light Microscope)
The ability of a light microscope to distinguish two close objects as separate, which is 250nm.
Magnification (Electron Microscope)
The degree to which an object is enlarged using an electron microscope, reaching up to 2,000,000X.
Micrometry
The scientific process of measuring the size of objects viewed under a microscope.
Ocular Micrometre
A glass disc marked with 100 equal divisions of arbitrary value, placed in the eye piece of a microscope.
Stage Micrometre
A glass slide containing an exact miniature scale used to calibrate the ocular micrometre.
Peptidoglycan
The chemical substance that makes up the cell walls of prokaryotes.
Chitin
The chemical substance that makes up the cell walls of fungi.
Middle Lamella
The layer present between the primary cell walls of adjacent cells, composed of magnesium and calcium salts of pectin.
Pectin
A polymer of approximately 200 galacturonic acid molecules that serves as a major component of the middle lamella.
Secondary Cell Wall
A thick, rigid layer formed between the primary wall and plasma membrane in sclerenchyma cells, containing cellulose, hemicelluloses, lignin, inorganic salts, and waxes.
Lignin
A substance in the secondary cell wall that cements cellulose microfibrils together and provides rigidity.
Plasma Membrane Composition
Chemically consists of proteins (60-80%), lipids (20-40%), and a small quantity of carbohydrates.
Fluid Mosaic Model
A model proposing the membrane is a phospholipid bilayer (7nm thick) with proteins scattered in an irregular pattern.
Cytosol
The less viscous, inner portion of the cytoplasm located towards the nucleus.
Cytogel
The more viscous, peripheral part of the cytoplasm located towards the plasma membrane.
Cyclosis
A circular streaming movement of the cytoplasm caused by microfilament activity, responsible for distributing cell contents.
Sarcoplasmic Reticulum
The name given to smooth endoplasmic reticulum specifically found in skeletal muscle cells.
80S Ribosomes
Large Ribosomes characteristic of eukaryotic cells, composed of 60S and 40S subunits.
70S Ribosomes
Smaller ribosomes found in prokaryotic cells, as well as in the mitochondria and chloroplasts of eukaryotic cells.
Polysome
A chain of several ribosomes attached to a single mRNA molecule during protein synthesis.
Cisternae
The stack of flattened, membrane-bound sacs that make up the Golgi complex and endoplasmic reticulum.
Cis Face
The convex "forming face" of the Golgi complex where new cisternae are created by fusing vesicles.
Trans Face
The concave "maturing face" of the Golgi complex where cisternae break up into vesicles.
Phragmoplast
A structure formed during plant cell cytokinesis by the fusion of Golgi vesicles, eventually giving rise to the new cell wall.
Primary Lysosomes
Newly formed, spherical vesicles containing hydrolytic enzymes before they begin their digestive function.
Autophagy
A "self-eating" process where lysosomes digest a cell's own unwanted structures or organelles.
Autolysis
A programmed cell death process where lysosomes burst, releasing enzymes that disintegrate the entire cell; also known as "suicidal bags."
Tay-Sachs Disease
A lysosomal storage disease caused by a missing lipid-digesting enzyme, leading to brain impairment from lipid accumulation.
Peroxisomes
Microbodies containing oxidative enzymes like catalase and peroxidase, primarily involved in the detoxification of alcohol and the breakdown of H2O2.
Glyoxysomes
Plant-specific microbodies present in oil-seed seedlings that convert stored fatty acids into carbohydrates via the glyoxylate cycle.
Tonoplast
The specialized membrane that separates the central vacuole from the surrounding cytoplasm in plant cells.
Porins
Special proteins in the outer mitochondrial and chloroplast membranes that allow free passage of various molecules.
Cristae
The inward folds of the inner mitochondrial membrane that increase surface area for ATP production.
Leucoplasts
Colourless plastids found in roots and seeds used for food storage, including amyloplasts, elaioplasts, and proteinoplasts.
Chromoplasts
Plastids that synthesize non-green pigments, found in flower petals and fruit walls to attract pollinators.
Granum
A stack of 25-50 smaller thylakoids found within the chloroplast; plural is grana.
Centrioles
Non-membranous organelles composed of nine triplets of microtubules, involved in forming spindle fibres and basal bodies.
Microfilaments
Thin contractile fibres (7nm diameter) made of actin, involved in cyclosis and muscle contraction.
Microtubules
Hollow cylinders (25nm diameter) composed of tubulin protein, forming centrioles, cilia, flagella, and spindle fibres.
Intermediate Filaments
Fibres (8-10nm diameter) composed of vimentin, providing mechanical support to the nuclear envelope and plasma membrane.
Axoneme
The internal bundle of eleven longitudinal microtubules in cilia and flagella, typically arranged in a "9+2" pattern.
Nucleoporin
A specialized transport protein that composes the nuclear pores and regulates exchange between the nucleus and cytoplasm.
Nucleolus
A non-membrane bound structure within the nucleoplasm responsible for the construction of ribosomes.
Kinetochores
A complex of proteins on opposite sides of the centromere that serves as the attachment site for microtubules during cell division.
Binary Fission
The simple method of cell division used by prokaryotic cells instead of mitosis or meiosis.