Summary of Intracellular metabolic reactions (ENDOENZYMES)

0.0(0)
Studied by 0 people
call kaiCall Kai
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
GameKnowt Play
Card Sorting

1/14

encourage image

There's no tags or description

Looks like no tags are added yet.

Last updated 2:51 AM on 4/15/26
Name
Mastery
Learn
Test
Matching
Spaced
Call with Kai

No analytics yet

Send a link to your students to track their progress

15 Terms

1
New cards

Carbohydrate Fermentation

Substrates: Glucose, Sucrose, Lactose

Enzymes: Misc., Sucrase, Lactase

Minimum Incubation: 24 hrs

Reagents: Phenol Red indicator

2
New cards

Carbohydrate Fermentation Test results:

Positive

  • Acid=yellow color

  • Gas = bubbles in inverted Durham tube

Negative

  • Alkaline=red color

  • No gas = no bubbles in tube

3
New cards

Citrate Utilization (slant tube)

Substrates: Citrate

Enzyme: Citrase

Minimum incubation: 48 hours

Reagents: Bromthmyol blue

4
New cards

Citrate Utilization Test Results:

Positive:

  • growth on slant and/or Prussian blue color

Negative

  • No growth and no color change

5
New cards

Methyl Red

Substrate: Glucose

Enzyme: Misc.

Incubation: 3-5 days

Reagents: Methyl Red indicator

6
New cards

Test results for Methyl Red:

Positive:

  • Mixed acid production (red color)

Negative

  • No acid production (any color other than red)

7
New cards

Voges-Proskauer (VP)

Substrate: Glucose

Enzymes: Misc.

Minimum Incubation time: 3-5 days

Reagents: Barritt’s A and Barritt’s B

8
New cards

Voges-Proskauer test results:

Positive:

  • Acetoin produced (red color)

Negative:

  • No acetoin produced (any color other than red)

9
New cards

Indole

Substrate: Tryptophan

Enzyme: Tryptophanase

Minimum incubation: 48 hrs

Reagents: Kovac’s reagent

10
New cards

Indole test results:

Positive:

  • Indole production (red ring of color at top of broth)

Negative

  • any color other than red in ring at top of broth

11
New cards

Nitrate Reduction

Substrate: Nitrate

Enzymes: Nitrate reductase

Minimum Incubation: 48 hrs

Reagents: Nitrite A and Nitrite B

12
New cards

Nitrate reduction test results:

Positive:

  • Red color after addition of reagents

Negative:

  • No red color after reagents but turns red after addition of zinc dust

13
New cards

Catalase:

Substrate: None

Enzyme: Catalase

Minimum Incubation: cultures at least 24 hours

Reagents: Hydrogen peroxide

14
New cards

Catalase test results:

Positive

  • Formation of bubbles after reagent

Negative:

  • no visible bubbles formed after reagent

15
New cards

Endoenzymes:

Carbohydrate Fermentation

Citrate Utilization

Methyl Red

Voges-Proskauer

Indole

Nitrate Reduction

Catalase